Vitamin D test cost in Florida
In Florida, 77 hospitals publish a cash price for Vitamin D blood test, 25-hydroxy (the standard vitamin D level test): from $14.00 to $580.82, with a median of $45.00. The lowest price is at Memorial Hospital Miramar (Miramar) and 3 other hospitals. The highest price is 41.5× the lowest.
Outpatient cash prices, one line per hospital — its lowest listed price for code 82306. Prices for both sides of the body and inpatient prices are not mixed in. Collected Sep 23, 2026.
Lowest$14.00Memorial Hospital Miramar + 3 more
Median$45.00half pay less
Highest$580.82in Florida
Lowest saves$31.00vs the median
Hospitals in Florida, cheapest first
| Hospital | Cash price | List price | File date |
|---|---|---|---|
| Memorial Hospital Miramar lowest Miramar · Miami-Fort Lauderdale-West Palm Beach, FL HC Cardio Iq« Vitamin D, 25-Hydroxy Quest #91735 | $14.00 | $20.00 | May 27, 2026 source file |
| Memorial Hospital Pembroke lowest Pembroke Pines · Miami-Fort Lauderdale-West Palm Beach, FL HC Cardio Iq« Vitamin D, 25-Hydroxy Quest #91735 | $14.00 | $20.00 | May 27, 2026 source file |
| Memorial Hospital West lowest Pembroke Pines · Miami-Fort Lauderdale-West Palm Beach, FL HC Cardio Iq« Vitamin D, 25-Hydroxy Quest #91735 | $14.00 | $20.00 | May 27, 2026 source file |
| Memorial Regional Hospital lowest Hollywood · Miami-Fort Lauderdale-West Palm Beach, FL HC Cardio Iq« Vitamin D, 25-Hydroxy Quest #91735 | $14.00 | $20.00 | May 27, 2026 source file |
| Boca Raton Regional Hospital Boca Raton · Miami-Fort Lauderdale-West Palm Beach, FL ARU VIT D, 25 HYDROXY | $23.40 | $36.00 | Mar 30, 2026 source file |
| OHI West, Inc. Saint Petersburg · Tampa-St. Petersburg-Clearwater, FL Hb Vitamin D | $24.00 | $60.00 | Apr 1, 2026 source file |
| Orlando Health Central, Inc. Ocoee · Orlando-Kissimmee-Sanford, FL Hb Vitamin D | $24.00 | $60.00 | Apr 1, 2026 source file |
| Orlando Health Lakeland Highlands Hospital Lakeland · Lakeland-Winter Haven, FL Hb Vitamin D | $24.00 | $60.00 | Apr 1, 2026 source file |
| Orlando Health Melbourne Hospital Melbourne · Palm Bay-Melbourne-Titusville, FL Hb Vitamin D | $24.00 | $60.00 | Apr 1, 2026 source file |
| Orlando Health Sebastian River Hospital Sebastian · Sebastian-Vero Beach-West Vero Corridor, FL Hb Vitamin D | $24.00 | $60.00 | Apr 1, 2026 source file |
| Orlando Health Wiregrass Ranch Hospital Wesley Chapel · Tampa-St. Petersburg-Clearwater, FL Hb Vitamin D | $24.00 | $60.00 | Apr 1, 2026 source file |
| Orlando Health, Inc. Saint Cloud · Orlando-Kissimmee-Sanford, FL Hb Vitamin D | $24.00 | $60.00 | Apr 1, 2026 source file |
| Orlando Health, Inc. Orlando · Orlando-Kissimmee-Sanford, FL Hb Vitamin D | $24.00 | $60.00 | Apr 1, 2026 source file |
| South Lake Hospital, Inc. Clermont · Orlando-Kissimmee-Sanford, FL Hb Vitamin D | $24.00 | $60.00 | Apr 1, 2026 source file |
| UF Health Leesburg · Orlando-Kissimmee-Sanford, FL UF HB 25-HYDROXY- D 2 AND D3, SERUM | $25.30 | $46.00 | Mar 31, 2026 source file |
| UF Health The Villages · Orlando-Kissimmee-Sanford, FL UF HB 25-HYDROXY- D 2 AND D3, SERUM | $25.30 | $46.00 | Mar 31, 2026 source file |
| University of Florida Health Gainesville · Gainesville, FL UF HB 25-HYDROXY- D 2 AND D3, SERUM | $25.30 | $46.00 | Mar 31, 2026 source file |
| Cape Coral Hospital Cape Coral · Cape Coral-Fort Myers, FL HC Lab Vitamin D, 25-Hydroxy | $30.80 | $154.00 | Mar 26, 2026 source file |
| Gulf Coast Medical Center Fort Myers · Cape Coral-Fort Myers, FL HC Lab Vitamin D, 25-Hydroxy | $30.80 | $154.00 | Mar 26, 2026 source file |
| Healthpark Medical Center Fort Myers · Cape Coral-Fort Myers, FL HC Lab Vitamin D, 25-Hydroxy | $30.80 | $154.00 | Mar 26, 2026 source file |
| Lee Memorial Hospital Fort Myers · Cape Coral-Fort Myers, FL HC Lab Vitamin D, 25-Hydroxy | $30.80 | $154.00 | Mar 26, 2026 source file |
| Rehab Hospital Fort Myers · Cape Coral-Fort Myers, FL HC Lab Vitamin D, 25-Hydroxy | $30.80 | $154.00 | Mar 26, 2026 source file |
| Cleveland Clinic Weston Hospital Nonprofit Corporation Weston · Miami-Fort Lauderdale-West Palm Beach, FL VIT D 25 HYDROXY ASSAY | $34.45 | $53.00 | Jun 15, 2026 source file |
| Indian River Memorial Hospital INC Vero Beach · Sebastian-Vero Beach-West Vero Corridor, FL VIT D 25 HYDROXY ASSAY | $34.45 | $53.00 | Jun 15, 2026 source file |
| Martin Memorial Medical Center INC Stuart · Port St. Lucie, FL VIT D 25 HYDROXY ASSAY | $34.45 | $53.00 | Jun 15, 2026 source file |
| Cape Canaveral Hospital Cocoa Beach · Palm Bay-Melbourne-Titusville, FL HC ASSAY OF VIT DCALCIFEDIOL W FRACTIONS IF PERF | $38.75 | $155.00 | Mar 2, 2026 source file |
| Holmes Regional Medical Center Melbourne · Palm Bay-Melbourne-Titusville, FL HC ASSAY OF VIT DCALCIFEDIOL W FRACTIONS IF PERFOR | $38.75 | $155.00 | Mar 2, 2026 source file |
| Palm Bay Hospital Palm Bay · Palm Bay-Melbourne-Titusville, FL HC ASSAY OF VIT DCALCIFEDIOL W FRACTIONS IF PERFOR | $38.75 | $155.00 | Mar 2, 2026 source file |
| Viera Hospital Melbourne · Palm Bay-Melbourne-Titusville, FL HC ASSAY OF VIT DCALCIFEDIOL W FRACTIONS IF PERFOR | $38.75 | $155.00 | Mar 2, 2026 source file |
| University of Florida Health in Jacksonville Jacksonville · Jacksonville, FL HB VITAMIN D 2+3 VIT D 25 HYDROXY | $44.32 | $80.58 | Mar 31, 2026 source file |
| Baptist Hospital of Miami Miami · Miami-Fort Lauderdale-West Palm Beach, FL ARU 25-Hydroxyvita D2 D3 By Tms | $44.85 | $69.00 | Mar 30, 2026 source file |
| Bethesda Hospital Boynton Beach · Miami-Fort Lauderdale-West Palm Beach, FL ARU 25-Hydroxyvita D2 D3 By Tms | $44.85 | $69.00 | Mar 30, 2026 source file |
| Doctors Hospital Coral Gables · Miami-Fort Lauderdale-West Palm Beach, FL ARU 25-Hydroxyvita D2 D3 By Tms | $44.85 | $69.00 | Mar 30, 2026 source file |
| Fishermens Health, INC Marathon · Key West-Key Largo, FL ARU 25-Hydroxyvita D2 D3 By Tms | $44.85 | $69.00 | Mar 30, 2026 source file |
| Homestead Hospital Homestead · Miami-Fort Lauderdale-West Palm Beach, FL ARU 25-Hydroxyvita D2 D3 By Tms | $44.85 | $69.00 | Mar 30, 2026 source file |
| Mariners Hospital Tavernier · Key West-Key Largo, FL ARU 25-Hydroxyvita D2 D3 By Tms | $44.85 | $69.00 | Mar 30, 2026 source file |
| South Miami Hospital South Miami · Miami-Fort Lauderdale-West Palm Beach, FL ARU 25-Hydroxyvita D2 D3 By Tms | $44.85 | $69.00 | Mar 30, 2026 source file |
| West Kendall Baptist Hospital Miami · Miami-Fort Lauderdale-West Palm Beach, FL ARU 25-Hydroxyvita D2 D3 By Tms | $44.85 | $69.00 | Mar 30, 2026 source file |
| AdventHealth New Smyrna Beach New Smyrna Beach · Deltona-Daytona Beach-Ormond Beach, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $45.00 | $45.00 | Apr 1, 2026 source file |
| AdventHealth Orlando Orlando · Orlando-Kissimmee-Sanford, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY - ARUP | $47.00 | $47.00 | Apr 1, 2026 source file |
| Sacred Heart Health System, Inc. Joe · Joe, FL 25-HYDROXYVITAMIN D2-D3 ESO | $49.33 | $123.32 | — source file |
| HMA Santa Rosa Medical Center LLC Milton · Pensacola-Ferry Pass-Brent, FL Vitamin D 25-Hydroxy LC | $50.06 | $333.72 | Apr 1, 2026 source file |
| AdventHealth Deland Deland · Deltona-Daytona Beach-Ormond Beach, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $52.50 | $52.50 | Apr 1, 2026 source file |
| Adventhealth Fish Memorial Orange City · Deltona-Daytona Beach-Ormond Beach, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $52.50 | $52.50 | Apr 1, 2026 source file |
| Adventhealth Palm Coast Palm Coast · Deltona-Daytona Beach-Ormond Beach, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $52.50 | $52.50 | Apr 1, 2026 source file |
| Adventhealth Palm Coast Parkway Palm Coast · Deltona-Daytona Beach-Ormond Beach, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $52.50 | $52.50 | Apr 1, 2026 source file |
| Adventhealth Waterman Tavares · Orlando-Kissimmee-Sanford, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $52.50 | $52.50 | Apr 1, 2026 source file |
| Sacred Heart Health System, Inc. Miramar Beach · Crestview-Fort Walton Beach-Destin, FL 25-HYDROXYVITAMIN D2-D3 ESO | $53.02 | $132.54 | — source file |
| Sacred Heart Health System, Inc. Pensacola · Pensacola-Ferry Pass-Brent, FL 25-HYDROXYVITAMIN D2-D3 ESO | $55.32 | $138.30 | — source file |
| Sacred Heart Health System, Inc. Panama City · Panama City-Panama City Beach, FL 25-HYDROXYVITAMIN D2-D3 ESO | $55.32 | $138.30 | — source file |
| Reunion Rehabilitation Hospital Jacksonville, LLC Jacksonville · Jacksonville, FL Vitamin d 25 hydroxy | $69.56 | $69.56 | Apr 1, 2026 source file |
| St Vincents Health System Inc Saint Johns · Jacksonville, FL VITAMIN D 25-OH LEVEL | $71.04 | $192.00 | — source file |
| St. Luke's-St. Vincent's Healthcare, Inc. Jacksonville · Jacksonville, FL VITAMIN D 25-OH LEVEL | $71.04 | $192.00 | — source file |
| St. Vincent's Medical Center, Inc. (FL) Jacksonville · Jacksonville, FL VITAMIN D 25-OH LEVEL | $71.04 | $192.00 | — source file |
| St. Vincent's Medical Center-Clay County, Inc. Middleburg · Jacksonville, FL VITAMIN D 25-OH LEVEL | $71.04 | $192.00 | — source file |
| Tampa General Hospital Tampa · Tampa-St. Petersburg-Clearwater, FL Hb Vitamin D | $76.65 | $219.00 | Apr 1, 2026 source file |
| Adventhealth Heart of Florida Davenport · Lakeland-Winter Haven, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $93.24 | $93.24 | Apr 1, 2026 source file |
| Adventhealth Lake Wales Lake Wales · Lakeland-Winter Haven, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $93.24 | $93.24 | Apr 1, 2026 source file |
| Adventhealth Dade City Dade City · Tampa-St. Petersburg-Clearwater, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $110.00 | $110.00 | Apr 1, 2026 source file |
| Adventhealth North Pinellas Tarpon Springs · Tampa-St. Petersburg-Clearwater, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $110.00 | $110.00 | Apr 1, 2026 source file |
| H. Lee Moffitt Cancer Center and Research Institute Hospital, Inc. Tampa · Tampa-St. Petersburg-Clearwater, FL Vitamin D (25-OH) | $115.50 | $231.00 | — source file |
| Adventhealth Carrollwood Tampa · Tampa-St. Petersburg-Clearwater, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $117.74 | $117.74 | Apr 1, 2026 source file |
| Adventhealth Riverview Riverview · Tampa-St. Petersburg-Clearwater, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $117.74 | $117.74 | Apr 1, 2026 source file |
| Adventhealth Tampa Tampa · Tampa-St. Petersburg-Clearwater, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $117.74 | $117.74 | Apr 1, 2026 source file |
| Adventhealth Wesley Chapel Wesley Chapel · Tampa-St. Petersburg-Clearwater, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $117.74 | $117.74 | Apr 1, 2026 source file |
| Adventhealth Zephyrhills Zephyrhills · Tampa-St. Petersburg-Clearwater, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $117.74 | $117.74 | Apr 1, 2026 source file |
| Holy Cross Hospital-Ft. Lauderdale Ft. Lauderdale · Miami-Fort Lauderdale-West Palm Beach, FL PR Vitamin D 25-Hydroxy | $164.00 | $164.00 | Mar 31, 2026 source file |
| Adventhealth Port Charlotte Port Charlotte · Punta Gorda, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $219.11 | $219.11 | Apr 1, 2026 source file |
| Broward Health Imperial Point Fort Lauderdale · Miami-Fort Lauderdale-West Palm Beach, FL HC ASSAY OF VIT D,CALCIFEDIOL W FRACTIONS, IF PERFORMED - VITAMIN D 25 | $297.51 | $297.51 | Sep 15, 2026 source file |
| Adventhealth Wauchula Wauchula · Wauchula, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $299.71 | $299.71 | Apr 1, 2026 source file |
| Adventhealth Sebring Sebring · Sebring, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $308.84 | $308.84 | Apr 1, 2026 source file |
| Adventhealth Daytona Beach Daytona Beach · Deltona-Daytona Beach-Ormond Beach, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $343.69 | $343.69 | Apr 1, 2026 source file |
| Broward Health Coral Springs Coral Springs · Miami-Fort Lauderdale-West Palm Beach, FL HC ASSAY OF VIT D,CALCIFEDIOL W FRACTIONS, IF PERFORMED - VITAMIN D 25 | $377.39 | $377.39 | Sep 15, 2026 source file |
| Broward Health North Deerfield Beach · Miami-Fort Lauderdale-West Palm Beach, FL HC ASSAY OF VIT D,CALCIFEDIOL W FRACTIONS, IF PERFORMED - VITAMIN D 25 | $380.86 | $380.86 | Sep 15, 2026 source file |
| Adventhealth Connerton Land O Lakes · Tampa-St. Petersburg-Clearwater, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $391.53 | $391.53 | Apr 1, 2026 source file |
| Broward Health Medical Center Fort Lauderdale · Miami-Fort Lauderdale-West Palm Beach, FL HC ASSAY OF VIT D,CALCIFEDIOL W FRACTIONS, IF PERFORMED - VITAMIN D 25 | $395.91 | $395.91 | Sep 15, 2026 source file |
| Adventhealth Ocala Ocala · Ocala, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $580.82 | $580.82 | Apr 1, 2026 source file |
By metro area
Each metro page compares every listing, including prices for both sides of the body.
| Metro area | Hospitals | Lowest | Median |
|---|---|---|---|
| Miami-Fort Lauderdale-West Palm Beach, FL | 17 | $14.00 | $44.85 |
| Tampa-St. Petersburg-Clearwater, FL | 12 | $24.00 | $116.62 |
| Orlando-Kissimmee-Sanford, FL | 8 | $24.00 | $24.65 |
| Deltona-Daytona Beach-Ormond Beach, FL | 6 | $45.00 | $52.50 |
| Jacksonville, FL | 6 | $44.32 | $71.04 |
| Cape Coral-Fort Myers, FL | 5 | $30.80 | $30.80 |
| Palm Bay-Melbourne-Titusville, FL | 5 | $24.00 | $38.75 |
| Lakeland-Winter Haven, FL | 3 | $24.00 | $93.24 |
| Key West-Key Largo, FL | 2 | $44.85 | $44.85 |
| Pensacola-Ferry Pass-Brent, FL | 2 | $50.06 | $52.69 |
| Sebastian-Vero Beach-West Vero Corridor, FL | 2 | $24.00 | $29.22 |
| Crestview-Fort Walton Beach-Destin, FL | 1 | $53.02 | $53.02 |
| Gainesville, FL | 1 | $25.30 | $25.30 |
| Joe, FL | 1 | $49.33 | $49.33 |
| Ocala, FL | 1 | $580.82 | $580.82 |
| Panama City-Panama City Beach, FL | 1 | $55.32 | $55.32 |
| Port St. Lucie, FL | 1 | $34.45 | $34.45 |
| Punta Gorda, FL | 1 | $219.11 | $219.11 |
| Sebring, FL | 1 | $308.84 | $308.84 |
| Wauchula, FL | 1 | $299.71 | $299.71 |
What it is
This blood test measures 25-hydroxy vitamin D, the form doctors use to judge whether the body has enough vitamin D. It is ordered to check for deficiency, for example with bone loss, certain digestive conditions or while taking supplements.
What the price covers
The price usually covers the vitamin D test only. The blood draw is often billed separately.
Vitamin D blood test, 25-hydroxy (the standard vitamin D level test) in other states
- Alabama$273.00
- Alaska$114.30
- Arizona$51.04
- Arkansas$77.00
- California$63.96
- Colorado$54.82
- Connecticut$100.65
- Delaware$267.63
- Georgia$241.00
- Hawaii$81.00
- Idaho$32.81
- Illinois$183.60
- Indiana$134.75
- Iowa$96.80
- Kansas$222.95
- Kentucky$343.75
- Louisiana$68.42
- Maine$81.18
- Maryland$41.17
- Massachusetts$109.85
- Michigan$91.00
- Minnesota$96.80
- Mississippi$48.48
- Missouri$129.63
- Montana$88.35
- Nebraska$89.09
- Nevada$110.25
- New Hampshire$143.98
- New Jersey$34.04
- New Mexico$115.20
- New York$89.00
- North Carolina$168.00
- North Dakota$76.38
- Ohio$76.95
- Oklahoma$131.17
- Oregon$88.50
- Pennsylvania$149.40
- Puerto Rico$47.70
- Rhode Island$145.00
- South Carolina$151.45
- South Dakota$96.80
- Tennessee$52.05
- Texas$49.20
- Utah$84.70
- Vermont$212.00
- Virginia$118.40
- Washington$137.20
- West Virginia$71.50
- Wisconsin$150.00
- Wyoming$57.14
Median cash price across the state's hospitals.