Vitamin D test cost in Tampa, FL
In Tampa-St. Petersburg-Clearwater, FL, 12 hospitals list a cash price for Vitamin D blood test, 25-hydroxy (the standard vitamin D level test): from $24.00 to $391.53, with a median of $116.62. The lowest listed price is at Orlando Health Wiregrass Ranch Hospital and 1 other hospital — $92.62 less than the median here. Across Florida, the median is $45.00 at 77 hospitals.
Cash prices at 12 facilities across 8 cities for code 82306, as an outpatient — the setting a self-pay patient normally buys. Collected Sep 23, 2026; the hospital files themselves were last updated between Apr 1, 2026 and Apr 1, 2026.
Where each hospital's price falls
Each dot is one hospital's cash price on a scale from the lowest to the highest in Tampa-St. Petersburg-Clearwater, FL.
Hospitals, cheapest first
| Hospital | Cash price | List price | Insurers pay | Off list | File date |
|---|---|---|---|---|---|
| Orlando Health Wiregrass Ranch Hospital lowest Wesley Chapel, FL Hb Vitamin D | $24.00 | $60.00 | — | 60% | Apr 1, 2026 source file |
| OHI West, Inc. lowest Saint Petersburg, FL Hb Vitamin D | $24.00 | $60.00 | — | 60% | Apr 1, 2026 source file |
| Tampa General Hospital Tampa, FL Hb Vitamin D | $76.65 | $219.00 | — | 65% | Apr 1, 2026 source file |
| Adventhealth North Pinellas Tarpon Springs, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $110.00 | $110.00 | — | — | Apr 1, 2026 source file |
| Adventhealth North Pinellas Tarpon Springs, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $110.00 | $110.00 | — | — | Apr 1, 2026 source file |
| Adventhealth Dade City Dade City, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $110.00 | $110.00 | — | — | Apr 1, 2026 source file |
| Adventhealth Dade City Dade City, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $110.00 | $110.00 | — | — | Apr 1, 2026 source file |
| H. Lee Moffitt Cancer Center and Research Institute Hospital, Inc. Tampa, FL VIT D 25OH, OUTSIDE FACILITY | $115.50 | $231.00 | $43.66–$231.00 | 50% | — source file |
| H. Lee Moffitt Cancer Center and Research Institute Hospital, Inc. Tampa, FL VITAMIN D3 | $115.50 | $231.00 | $43.66–$231.00 | 50% | — source file |
| H. Lee Moffitt Cancer Center and Research Institute Hospital, Inc. Tampa, FL Vitamin D (25-OH) | $115.50 | $231.00 | $43.66–$231.00 | 50% | — source file |
| Adventhealth Tampa Tampa, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $117.74 | $117.74 | — | — | Apr 1, 2026 source file |
| Adventhealth Wesley Chapel Wesley Chapel, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $117.74 | $117.74 | — | — | Apr 1, 2026 source file |
| Adventhealth Riverview Riverview, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $117.74 | $117.74 | — | — | Apr 1, 2026 source file |
| Adventhealth Carrollwood Tampa, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY - ARUP | $117.74 | $117.74 | — | — | Apr 1, 2026 source file |
| Adventhealth Carrollwood Tampa, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $117.74 | $117.74 | — | — | Apr 1, 2026 source file |
| Adventhealth Carrollwood Tampa, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $117.74 | $117.74 | — | — | Apr 1, 2026 source file |
| Adventhealth Zephyrhills Zephyrhills, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $117.74 | $117.74 | — | — | Apr 1, 2026 source file |
| Adventhealth Zephyrhills Zephyrhills, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $117.74 | $117.74 | — | — | Apr 1, 2026 source file |
| Adventhealth Riverview Riverview, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $117.74 | $117.74 | — | — | Apr 1, 2026 source file |
| Adventhealth Wesley Chapel Wesley Chapel, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $117.74 | $117.74 | — | — | Apr 1, 2026 source file |
| Adventhealth Tampa Tampa, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $117.74 | $117.74 | — | — | Apr 1, 2026 source file |
| Adventhealth Connerton Land O Lakes, FL HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $391.53 | $391.53 | — | — | Apr 1, 2026 source file |
| Adventhealth Connerton Land O Lakes, FL HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $391.53 | $391.53 | — | — | Apr 1, 2026 source file |
Outpatient: lowest $24.00, median $116.62, highest $391.53 — a 16.3× difference within the same market.
As an inpatient, the same code is billed by 12 facilities here, median $116.62. Inpatient and outpatient prices are not comparable and are kept on separate pages: the inpatient charge covers admission, not the same purchase.
Cash or insurance?
At the one hospital here that publishes insurer rates for this code, the cash price is below the highest rate a health plan has negotiated. If you have insurance but have not met your deductible, you usually pay your plan's negotiated rate, which falls somewhere in the "Insurers pay" column. Paying cash can cost less, but a cash payment usually does not count toward your deductible. Ask the hospital for both numbers before you book.
What it is
This blood test measures 25-hydroxy vitamin D, the form doctors use to judge whether the body has enough vitamin D. It is ordered to check for deficiency, for example with bone loss, certain digestive conditions or while taking supplements.
What the price covers
The price usually covers the vitamin D test only. The blood draw is often billed separately.
2 rows held back from this comparison
They are priced below $1 or below a tenth of the national median for code 82306 ($112.00). Such a line is almost always a physician's fee billed separately, an assistant's share or a placeholder — a real charge, but not the price of the procedure itself.
| Orlando Health Wiregrass Ranch Hospital Wesley Chapel, FLHb Vitamin D 25-Hydroxy-Ref | $8.80 | file |
| OHI West, Inc. Saint Petersburg, FLHb Vitamin D 25-Hydroxy-Ref | $8.80 | file |