Vitamin D test cost in North Carolina
In North Carolina, 37 hospitals publish a cash price for Vitamin D blood test, 25-hydroxy (the standard vitamin D level test): from $15.00 to $281.00, with a median of $168.00. The lowest price is at Sentara Albemarle Regional Medical Center, LLC (Elizabeth City). The highest price is 18.7× the lowest.
Outpatient cash prices, one line per hospital — its lowest listed price for code 82306. Prices for both sides of the body and inpatient prices are not mixed in. Collected Sep 24, 2026.
Lowest$15.00Sentara Albemarle Regional Medical Center, LLC
Median$168.00half pay less
Highest$281.00in North Carolina
Lowest saves$153.00vs the median
Hospitals in North Carolina, cheapest first
| Hospital | Cash price | List price | File date |
|---|---|---|---|
| Sentara Albemarle Regional Medical Center, LLC lowest Elizabeth City · Elizabeth City, NC Chgl (Qu) Questassured? 25-Hydroxyvitamin D (D2, D3), S/O | $15.00 | $30.00 | Apr 1, 2026 source file |
| Pitt County Memorial Hospital, Inc. Washington · Washington, NC 92888 Vitamin D 25 Hydroxy D2 D3-Ref-Qu | $53.00 | $100.00 | Apr 1, 2026 source file |
| Pitt County Memorial Hospital, Inc. Greenville · Greenville, NC 92888 Vitamin D 25 Hydroxy D2 D3-Ref-Qu | $53.00 | $100.00 | Apr 1, 2026 source file |
| Duplin General Hospital, Inc. Kenansville · Kenansville, NC 92888 Vitamin D 25 Hydroxy D2 D3-Ref-Qu | $56.00 | $100.00 | Apr 1, 2026 source file |
| East Carolina Health Ahoskie · Ahoskie, NC 92888 Vitamin D 25 Hydroxy D2 D3-Ref-Qu | $56.00 | $100.00 | Apr 1, 2026 source file |
| East Carolina Health-Bertie Windsor · Windsor, NC 92888 Vitamin D 25 Hydroxy D2 D3-Ref-Qu | $56.00 | $100.00 | Apr 1, 2026 source file |
| East Carolina Health-Chowan, Inc. Edenton · Edenton, NC 92888 Vitamin D 25 Hydroxy D2 D3-Ref-Qu | $56.00 | $100.00 | Apr 1, 2026 source file |
| East Carolina Health-Heritage, Inc. Tarboro · Rocky Mount, NC 92888 Vitamin D 25 Hydroxy D2 D3-Ref-Qu | $56.00 | $100.00 | Apr 1, 2026 source file |
| Halifax Regional Medical Center, Inc. Roanoke Rapids · Roanoke Rapids, NC 92888 Vitamin D 25 Hydroxy D2 D3-Ref-Qu | $56.00 | $100.00 | Apr 1, 2026 source file |
| The Outer Banks Hospital, Inc. Nags Head · Kill Devil Hills, NC 92888 Vitamin D 25 Hydroxy D2 D3-Ref-Qu | $56.00 | $100.00 | Apr 1, 2026 source file |
| DUKE UNIVERSITY HEALTH SYSTEM, INC. Doing Business As: DUKE UNIVERSITY HOSPITAL Raleigh · Raleigh-Cary, NC Vitamin D 25 Hydroxy | $67.23 | $249.00 | — source file |
| DUKE UNIVERSITY HEALTH SYSTEM, INC. Doing Business As: DUKE UNIVERSITY HOSPITAL Durham · Durham, NC Vitamin D 25 Hydroxy | $67.23 | $249.00 | — source file |
| DUKE UNIVERSITY HEALTH SYSTEM, INC. Doing Business As: DURHAM REGIONAL HOSPITAL Durham · Durham-Chapel Hill, NC Vitamin D 25 Hydroxy | $67.23 | $249.00 | — source file |
| Lexington Medical Center Lexington · Winston-Salem, NC HC ASSAY OF VITAMIN D 25 | $112.00 | $224.00 | Oct 8, 2025 source file |
| WRMC Hospital Operating Corporation Elkin · Mount Airy, NC HC ASSAY OF VITAMIN D 25 | $139.00 | $278.00 | Jul 16, 2026 source file |
| WRMC Hospital Operating Corporation North Wilkesboro · North Wilkesboro, NC HC ASSAY OF VITAMIN D 25 | $139.00 | $278.00 | Oct 8, 2025 source file |
| North Carolina Baptist Hospital Bermuda Run · Winston-Salem, NC HC ASSAY OF VITAMIN D 25 | $159.00 | $318.00 | Oct 21, 2025 source file |
| Murphy Medical Center INC Murphy · Murphy, NC HC 25-Hydroxy Vitamin D | $163.80 | $234.00 | Jan 25, 2026 source file |
| High Point Regional Health High Point · Greensboro-High Point, NC HC ASSAY OF VITAMIN D 25 | $168.00 | $336.00 | Oct 8, 2025 source file |
| North Carolina Baptist Hospital Winston Salem · Winston-Salem, NC HC ASSAY OF VITAMIN D 25 | $168.00 | $336.00 | Oct 8, 2025 source file |
| Adventhealth Hendersonville Hendersonville · Asheville, NC HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $208.00 | $208.00 | Apr 1, 2026 source file |
| Atrium Health Hospitals Inc Wadesboro · Charlotte-Concord-Gastonia, NC-SC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | Dec 1, 2025 source file |
| Atrium Health Hospitals Inc Shelby · Shelby-Kings Mountain, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | Dec 1, 2025 source file |
| Atrium Health Hospitals Inc Lincolnton · Charlotte-Concord-Gastonia, NC-SC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | Dec 1, 2025 source file |
| Carolinas Medical Center Charlotte · Charlotte-Concord-Gastonia, NC-SC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | Dec 4, 2025 source file |
| Carolinas Rehabilitation Charlotte · Charlotte-Concord-Gastonia, NC-SC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | Dec 5, 2025 source file |
| Carolinas Rehabilitation Belmont · Charlotte-Concord-Gastonia, NC-SC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | Dec 5, 2025 source file |
| Carolinas Rehabilitation Concord · Concord, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | Dec 5, 2025 source file |
| The Charlotte Mecklenburg Hospital Authority Concord · Charlotte-Concord-Gastonia, NC-SC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | Dec 1, 2025 source file |
| The Charlotte Mecklenburg Hospital Authority Charlotte · Charlotte-Concord-Gastonia, NC-SC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | Dec 5, 2025 source file |
| The Charlotte Mecklenburg Hospital Authority Monroe · Charlotte-Concord-Gastonia, NC-SC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | Dec 4, 2025 source file |
| DUKE HEALTH LAKE NORMAN REGIONAL MEDICAL CENTER, INC. Doing Business As: DUKE HEALTH LAKE NORMAN HOSPITAL Mooresville · Charlotte-Concord-Gastonia, NC-SC Vitamin D 25 Hydroxy | $229.77 | $851.00 | — source file |
| Atrium Health Hospitals Inc Albermarle · Albemarle, NC HC ASSAY OF VITAMIN D 25 | $234.00 | $468.00 | Sep 15, 2026 source file |
| AdventHealth Polk Columbus · Columbus, NC HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $265.34 | $265.34 | Apr 1, 2026 source file |
| Gaston Memorial Hospital Gastonia · Charlotte-Concord-Gastonia, NC-SC CHG VITAMIN D 25 HYDROXY | $281.00 | $281.00 | May 11, 2026 source file |
| Gaston Memorial Hospital Mount Holly · Charlotte-Concord-Gastonia, NC-SC CHG VITAMIN D 25 HYDROXY | $281.00 | $281.00 | May 11, 2026 source file |
| Gaston Memorial Hospital Belmont · Charlotte-Concord-Gastonia, NC-SC CHG VITAMIN D 25 HYDROXY | $281.00 | $281.00 | May 11, 2026 source file |
By metro area
Each metro page compares every listing, including prices for both sides of the body.
| Metro area | Hospitals | Lowest | Median |
|---|---|---|---|
| Charlotte-Concord-Gastonia, NC-SC | 12 | $224.53 | $224.53 |
| Winston-Salem, NC | 3 | $112.00 | $159.00 |
| Ahoskie, NC | 1 | $56.00 | $56.00 |
| Albemarle, NC | 1 | $234.00 | $234.00 |
| Asheville, NC | 1 | $208.00 | $208.00 |
| Columbus, NC | 1 | $265.34 | $265.34 |
| Concord, NC | 1 | $224.53 | $224.53 |
| Durham, NC | 1 | $67.23 | $67.23 |
| Durham-Chapel Hill, NC | 1 | $67.23 | $67.23 |
| Edenton, NC | 1 | $56.00 | $56.00 |
| Elizabeth City, NC | 1 | $15.00 | $15.00 |
| Greensboro-High Point, NC | 1 | $168.00 | $168.00 |
| Greenville, NC | 1 | $53.00 | $53.00 |
| Kenansville, NC | 1 | $56.00 | $56.00 |
| Kill Devil Hills, NC | 1 | $56.00 | $56.00 |
| Murphy, NC | 1 | $163.80 | $163.80 |
| Mount Airy, NC | 1 | $139.00 | $139.00 |
| North Wilkesboro, NC | 1 | $139.00 | $139.00 |
| Raleigh-Cary, NC | 1 | $67.23 | $67.23 |
| Roanoke Rapids, NC | 1 | $56.00 | $56.00 |
| Rocky Mount, NC | 1 | $56.00 | $56.00 |
| Shelby-Kings Mountain, NC | 1 | $224.53 | $224.53 |
| Windsor, NC | 1 | $56.00 | $56.00 |
| Washington, NC | 1 | $53.00 | $53.00 |
What it is
This blood test measures 25-hydroxy vitamin D, the form doctors use to judge whether the body has enough vitamin D. It is ordered to check for deficiency, for example with bone loss, certain digestive conditions or while taking supplements.
What the price covers
The price usually covers the vitamin D test only. The blood draw is often billed separately.
Vitamin D blood test, 25-hydroxy (the standard vitamin D level test) in other states
- Alabama$273.00
- Alaska$114.30
- Arizona$51.04
- Arkansas$77.00
- California$63.96
- Colorado$54.82
- Connecticut$100.65
- Delaware$267.63
- Florida$45.00
- Georgia$241.00
- Hawaii$81.00
- Idaho$32.81
- Illinois$183.60
- Indiana$134.75
- Iowa$96.80
- Kansas$222.95
- Kentucky$343.75
- Louisiana$68.42
- Maine$81.18
- Maryland$41.17
- Massachusetts$109.85
- Michigan$91.00
- Minnesota$96.80
- Mississippi$48.48
- Missouri$129.63
- Montana$88.35
- Nebraska$89.09
- Nevada$110.25
- New Hampshire$143.98
- New Jersey$34.04
- New Mexico$115.20
- New York$89.00
- North Dakota$76.38
- Ohio$76.95
- Oklahoma$131.17
- Oregon$88.50
- Pennsylvania$149.40
- Puerto Rico$47.70
- Rhode Island$145.00
- South Carolina$151.45
- South Dakota$96.80
- Tennessee$52.05
- Texas$49.20
- Utah$84.70
- Vermont$212.00
- Virginia$118.40
- Washington$137.20
- West Virginia$71.50
- Wisconsin$150.00
- Wyoming$57.14
Median cash price across the state's hospitals.