Vitamin D test cost in Pennsylvania
In Pennsylvania, 65 hospitals publish a cash price for vitamin D blood test, 25-hydroxy (the standard vitamin D level test): from $13.00 to $532.20, with a median of $149.40. The lowest price is at Uniontown Hospital (Uniontown). The highest price is 40.9× the lowest.
Outpatient cash prices, one line per hospital — its lowest listed price for code 82306. Prices for both sides of the body and inpatient prices are not mixed in. Collected Sep 23, 2026.
Lowest$13.00Uniontown Hospital
Median$149.40half pay less
Highest$532.20in Pennsylvania
Lowest saves$136.40vs the median
Hospitals in Pennsylvania, cheapest first
| Hospital | Cash price | List price | File date |
|---|---|---|---|
| Uniontown Hospital lowest Uniontown · Pittsburgh, PA VITAMIN D 25 HYDROXY | $13.00 | $26.00 | — source file |
| AHN Wexford Hospital Wexford · Pittsburgh, PA CHG 25 HYDROXY INCLUDES FRACTIONS IF PERFORMED | $22.88 | $88.00 | Apr 14, 2026 source file |
| Lower Bucks Hospital Bristol · Philadelphia-Camden-Wilmington, PA-NJ-DE-MD Hc Assay Vit D, 25-Hydroxy W Fractions | $29.00 | $512.00 | Sep 1, 2026 source file |
| Roxborough Memorial Hospital Philadelphia · Philadelphia-Camden-Wilmington, PA-NJ-DE-MD Hc Assay Vit D, 25-Hydroxy W Fractions | $29.00 | $512.38 | Sep 1, 2026 source file |
| Pennsylvania Psychiatric Institute Harrisburg · Harrisburg-Carlisle, PA 82306 Vitamin D 25 Hydroxy Level. Tech | $32.95 | $192.00 | — source file |
| Penn State Health Holy Spirit Medical Center Camp Hill · Harrisburg-Carlisle, PA 25-Hydroxyvitamin D2 and D3, Serum Tech | $45.87 | $192.00 | — source file |
| St Joseph Regional Health Network Reading · Reading, PA Vitamin D 25 Hydroxy Level. | $49.94 | $192.00 | — source file |
| Troy Community Hospital Troy · Sayre, PA Questassured Vitamin D | $53.80 | $67.25 | — source file |
| Penn State Health Lancaster Medical Center Lancaster · Lancaster, PA Total 25 OH Vitamin D serum. Tech | $55.66 | $192.00 | — source file |
| Penn State Health Hampden Medical Center Enola · Harrisburg-Carlisle, PA 82306 QuestAssureD For Infants, 25-Hydroxyvitamin D -Quest Tech | $60.42 | $192.00 | — source file |
| Penn State Health Milton S. Hershey Medical Center Hershey · Harrisburg-Carlisle, PA 25-Hydroxyvitamin D2 and D3, Serum | $63.61 | $192.00 | — source file |
| Bryn Mawr Rehabilitation Hospital Malvern · Philadelphia-Camden-Wilmington, PA-NJ-DE-MD Hc Vitamin D, 25-Hydroxy | $64.80 | $162.00 | — source file |
| Lankenau Medical Center Wynnewood · Philadelphia-Camden-Wilmington, PA-NJ-DE-MD Hc Vitamin D, 25-Hydroxy | $64.80 | $162.00 | Apr 1, 2026 source file |
| Paoli Hospital Paoli · Philadelphia-Camden-Wilmington, PA-NJ-DE-MD Hc Vitamin D, 25-Hydroxy | $64.80 | $162.00 | Apr 1, 2026 source file |
| Riddle Memorial Hospital Media · Philadelphia-Camden-Wilmington, PA-NJ-DE-MD Hc Vitamin D, 25-Hydroxy | $64.80 | $162.00 | Apr 1, 2026 source file |
| The Bryn Mawr Hospital Bryn Mawr · Philadelphia-Camden-Wilmington, PA-NJ-DE-MD Hc Vitamin D, 25-Hydroxy | $64.80 | $162.00 | — source file |
| Punxsutawney Area Hospital Punxsutawney · Punxsutawney, PA 25 HYDROXY VITIMIN D | $75.96 | $211.00 | Mar 2, 2026 source file |
| UPMC Greene Waynesburg · Waynesburg, PA VITAMIN D (25 HYDROXYO | $94.29 | $134.70 | — source file |
| UPMC Washington Washington · Pittsburgh, PA VITAMIN D (25 HYDROXYO | $94.29 | $134.70 | — source file |
| Chambersburg Hospital Chambersburg · Chambersburg, PA Vitamin D-3 Level | $100.00 | $224.00 | — source file |
| Ephrata Community Hospital Ephrata · Lancaster, PA Vitamin D-3 Level | $100.00 | $154.00 | — source file |
| Evangelical Community Hospital Lewisburg · Lewisburg, PA Vitamin D-3 Level | $100.00 | $359.00 | — source file |
| Gettysburg Hospital Gettysburg · Gettysburg, PA Vitamin D-3 Level | $100.00 | $151.00 | — source file |
| Good Samaritan Hospital Lebanon · Lebanon, PA Vitamin D-3 Level | $100.00 | $154.00 | — source file |
| Waynesboro Hospital Waynesboro · Chambersburg, PA Vitamin D-3 Level | $100.00 | $224.00 | — source file |
| WellSpan Surgery and Rehabilitation Hospital York · York-Hanover, PA Vitamin D-3 Level | $100.00 | $151.00 | — source file |
| UPMC Jameson New Castle · Pittsburgh, PA VITAMIN D/25-HYDROXY | $100.20 | $167.00 | — source file |
| The Children's Hospital of Philadelphia Philadelphia · Philadelphia-Camden-Wilmington, PA-NJ-DE-MD Vitamin D, Serum-Arup | $103.54 | $103.54 | — source file |
| UPMC Bedford Everett · Everett, PA VITAMIN D/25-HYDROXY | $141.00 | $235.00 | — source file |
| UPMC Northwest Seneca · Oil City, PA CARD IQ VIT D 25HYDRX LC/MS/MS | $145.80 | $243.00 | — source file |
| UPMC Cole Coudersport · Coudersport, PA VITAMIN D - LC SCREENING | $147.00 | $245.00 | — source file |
| UPMC Carlisle Carlisle · Harrisburg-Carlisle, PA HC VITAMIN D 25-HYDROXY | $149.40 | $249.00 | — source file |
| UPMC Hanover Hanover · York-Hanover, PA HC VITAMIN D 25-HYDROXY | $149.40 | $249.00 | — source file |
| UPMC Lititz Lititz · Lancaster, PA HC VITAMIN D 25-HYDROXY | $149.40 | $249.00 | — source file |
| UPMC Memorial York · York-Hanover, PA HC VITAMIN D 25-HYDROXY | $149.40 | $249.00 | — source file |
| UPMC Pinnacle Hospitals Harrisburg · Harrisburg-Carlisle, PA HC VITAMIN D 25-HYDROXY | $149.40 | $249.00 | — source file |
| UPMC Somerset Somerset · Somerset, PA HC VITAMIN D 25-HYDROXY | $149.40 | $249.00 | — source file |
| UPMC Kane Kane · Bradford, PA VITAMIN D 25 OH | $160.20 | $267.00 | — source file |
| UPMC Presbyterian Pittsburgh · Pittsburgh, PA VITAMIN D/25-HYDROXY | $169.20 | $282.00 | Mar 6, 2026 source file |
| UPMC Childrens Hospital of Pittsburgh Pittsburgh · Pittsburgh, PA 25-OH VITAMIN D3 P3433 | $194.40 | $324.00 | Mar 6, 2026 source file |
| UPMC Magee-Womens Hospital Pittsburgh · Pittsburgh, PA HC VITAMIN D 25 HYDROXY INC FRACTION | $205.80 | $343.00 | — source file |
| Highlands Hospital Connellsville · Pittsburgh, PA VITAMIN D LEVEL | $208.80 | $348.00 | — source file |
| Monogahela Valley Hospital Monongahela · Pittsburgh, PA 25 HYDROXY VIT D | $208.80 | $348.00 | — source file |
| UPMC Horizon - Greenville Greenville · Hermitage, PA VITAMIN D IMMUNOASSAY | $222.60 | $371.00 | — source file |
| UPMC Mercy Pittsburgh · Pittsburgh, PA 25-OH VITAMIN D3 P3433 | $223.80 | $373.00 | — source file |
| UPMC Muncy Muncy · Williamsport, PA 25-HYDROXY-VITAMIN-D | $226.80 | $378.00 | — source file |
| UPMC Wellsboro Wellsboro · Wellsboro, PA VITAMIN D IMMUNOASSAY | $226.80 | $378.00 | — source file |
| UPMC Williamsport Williamsport · Williamsport, PA 25-OH VITAMIN D3 P3433 | $226.80 | $378.00 | — source file |
| Robert Packer Hospital Sayre · Sayre, PA Questassured Vitamin D | $237.12 | $296.40 | — source file |
| UPMC St Margaret Pittsburgh · Pittsburgh, PA 25-OH VITAMIN D3 P3433 | $250.80 | $418.00 | — source file |
| Shriners Childrens - Philadelphia Philadelphia · Philadelphia-Camden-Wilmington, PA-NJ-DE-MD VITAMIN D-3 LEVEL | $261.50 | $261.50 | — source file |
| UPMC McKeesport McKeesport · Pittsburgh, PA CARD IQ VIT D 25HYDRX LC/MS/MS | $265.20 | $442.00 | — source file |
| UPMC Altoona Altoona · Altoona, PA 25-HYDROXY-VITAMIN-D | $271.80 | $453.00 | — source file |
| St Mary Medical Center Langhorne · Philadelphia-Camden-Wilmington, PA-NJ-DE-MD HC Quest Vitamin D 25-Hydroxy D2/D3 | $310.05 | $477.00 | Mar 31, 2026 source file |
| Mercy Fitzgerald Hospital Darby · Philadelphia-Camden-Wilmington, PA-NJ-DE-MD HC Vitamin D 25-Hydroxy | $311.35 | $479.00 | Mar 31, 2026 source file |
| Nazareth Hospital Philadelphia · Philadelphia-Camden-Wilmington, PA-NJ-DE-MD HC Vitamin D 25-Hydroxy | $313.95 | $483.00 | Mar 31, 2026 source file |
| Robert Packer Hospital, Towanda Campus Towanda · Sayre, PA Questassured Vitamin D | $328.64 | $410.80 | — source file |
| Penn Highlands Brookville Brookville · Brookville, PA Vit D | $348.00 | $348.00 | Apr 1, 2026 source file |
| Penn Highlands Dubois DuBois · DuBois, PA Cardio IQ Vitamin D, 25-Hydroxy- Quest | $348.00 | $348.00 | Aug 1, 2025 source file |
| Penn Highlands Elk Saint Marys · St. Marys, PA Cardio IQ Vitamin D, 25-Hydroxy- Quest | $348.00 | $348.00 | Apr 1, 2026 source file |
| Penn Highlands Huntingdon Huntingdon · Huntingdon, PA Vit D | $348.00 | $348.00 | Apr 1, 2026 source file |
| Penn Highlands Tyrone Tyrone · Altoona, PA Cardio IQ Vitamin D, 25-Hydroxy- Quest | $348.00 | $348.00 | Apr 1, 2026 source file |
| UPMC East Monroeville · Pittsburgh, PA 25-OH VITAMIN D3 P3433 | $441.60 | $736.00 | — source file |
| UPMC Hamot Erie · Erie, PA 25-OH VITAMIN D3 P3433 | $501.00 | $835.00 | — source file |
| UPMC Passavant - McCandless Pittsburgh · Pittsburgh, PA HC VITAMIN D 25 HYDROXY INC FRACTION | $532.20 | $887.00 | — source file |
By metro area
Each metro page compares every listing, including prices for both sides of the body.
| Metro area | Hospitals | Lowest | Median |
|---|---|---|---|
| Pittsburgh, PA | 14 | $13.00 | $207.30 |
| Philadelphia-Camden-Wilmington, PA-NJ-DE-MD | 12 | $29.00 | $64.80 |
| Harrisburg-Carlisle, PA | 6 | $32.95 | $62.02 |
| Lancaster, PA | 3 | $55.66 | $100.00 |
| Sayre, PA | 3 | $53.80 | $237.12 |
| York-Hanover, PA | 3 | $100.00 | $149.40 |
| Altoona, PA | 2 | $271.80 | $309.90 |
| Chambersburg, PA | 2 | $100.00 | $100.00 |
| Williamsport, PA | 2 | $226.80 | $226.80 |
| Brookville, PA | 1 | $348.00 | $348.00 |
| Bradford, PA | 1 | $160.20 | $160.20 |
| Coudersport, PA | 1 | $147.00 | $147.00 |
| DuBois, PA | 1 | $348.00 | $348.00 |
| Everett, PA | 1 | $141.00 | $141.00 |
| Erie, PA | 1 | $501.00 | $501.00 |
| Gettysburg, PA | 1 | $100.00 | $100.00 |
| Hermitage, PA | 1 | $222.60 | $222.60 |
| Huntingdon, PA | 1 | $348.00 | $348.00 |
| Lebanon, PA | 1 | $100.00 | $100.00 |
| Lewisburg, PA | 1 | $100.00 | $100.00 |
| Oil City, PA | 1 | $145.80 | $145.80 |
| Punxsutawney, PA | 1 | $75.96 | $75.96 |
| Reading, PA | 1 | $49.94 | $49.94 |
| Somerset, PA | 1 | $149.40 | $149.40 |
| St. Marys, PA | 1 | $348.00 | $348.00 |
| Waynesburg, PA | 1 | $94.29 | $94.29 |
| Wellsboro, PA | 1 | $226.80 | $226.80 |
What it is
This blood test measures 25-hydroxy vitamin D, the form doctors use to judge whether the body has enough vitamin D. It is ordered to check for deficiency, for example with bone loss, certain digestive conditions or while taking supplements.
What the price covers
The price usually covers the vitamin D test only. The blood draw is often billed separately.
Vitamin D blood test, 25-hydroxy (the standard vitamin D level test) in other states
- Alabama$273.00
- Alaska$114.30
- Arizona$51.04
- Arkansas$77.00
- California$63.96
- Colorado$54.82
- Connecticut$100.65
- Delaware$267.63
- Florida$45.00
- Georgia$241.00
- Hawaii$81.00
- Idaho$32.81
- Illinois$183.60
- Indiana$134.75
- Iowa$96.80
- Kansas$222.95
- Kentucky$343.75
- Louisiana$68.42
- Maine$81.18
- Maryland$41.17
- Massachusetts$109.85
- Michigan$91.00
- Minnesota$96.80
- Mississippi$48.48
- Missouri$129.63
- Montana$88.35
- Nebraska$89.09
- Nevada$110.25
- New Hampshire$143.98
- New Jersey$34.04
- New Mexico$115.20
- New York$89.00
- North Carolina$168.00
- North Dakota$76.38
- Ohio$76.95
- Oklahoma$131.17
- Oregon$88.50
- Puerto Rico$47.70
- Rhode Island$145.00
- South Carolina$151.45
- South Dakota$96.80
- Tennessee$52.05
- Texas$49.20
- Utah$84.70
- Vermont$212.00
- Virginia$118.40
- Washington$137.20
- West Virginia$71.50
- Wisconsin$150.00
- Wyoming$57.14
Median cash price across the state's hospitals.