Vitamin D test cost in Orlando, FL
In Orlando-Kissimmee-Sanford, FL, 13 hospitals list a cash price for vitamin D blood test, 25-hydroxy (the standard vitamin D level test): from $11.95 to $52.97, with a median of $25.30. The lowest listed price is at HCA Florida Osceola Hospital — $13.35 less than the median here. Across Florida, the median is $46.00 at 164 hospitals.
- The median here, $25.30, is 78% below the US median of $114.80.
- That is 0.9× Medicare's 2026 national rate for the same service ($29.60).
- Orlando, FL has the lowest median of 3 metro areas in Florida with at least 10 hospitals pricing this code.
- 7 of 13 hospitals list a cash price equal to their full list price, with no self-pay discount.
Cash prices at 13 facilities across 8 cities for code 82306, as an outpatient — the setting a self-pay patient normally buys. Collected Oct 31, 2026; the hospital files themselves were last updated between Mar 10, 2026 and Sep 1, 2026.
Where each hospital's price falls
Each dot is one hospital's cash price on a scale from the lowest to the highest in Orlando-Kissimmee-Sanford, FL.
Hospitals, cheapest first
| Hospital | Cash price | List price | Insurers pay | Off list |
|---|---|---|---|---|
| HCA Florida Osceola Hospital lowest Orlando, FL · (407) 846-2266 VITAMIN D3 25-OH File of Sep 1, 2026 · source file | $11.95 | $11.95 | $0.86–$11.95 | — |
| South Lake Hospital Clermont, FL · (352) 394-4071 Hb Vitamin D File of Apr 1, 2026 · source file | $24.00 | $60.00 | — | 60% |
| Orlando Health Orlando, FL · (321) 841-5111 Hb Vitamin D File of Apr 1, 2026 · source file | $24.00 | $60.00 | — | 60% |
| Orlando Health Central Ocoee, FL · (407) 296-1000 Hb Vitamin D File of Apr 1, 2026 · source file | $24.00 | $60.00 | — | 60% |
| Orlando Health St Cloud Hospital Saint Cloud, FL · (407) 892-2135 Hb Vitamin D File of Apr 1, 2026 · source file | $24.00 | $60.00 | — | 60% |
| The Villages Regional Hospital The Villages, FL · (352) 751-8000 UF HB 25-HYDROXY- D 2 AND D3, SERUM File of Mar 31, 2026 · source file | $25.30 | $46.00 | $20.70–$38.18 | 45% |
| UF Health Leesburg Hospital Leesburg, FL · (352) 323-5762 UF HB 25-HYDROXY- D 2 AND D3, SERUM File of Mar 31, 2026 · source file | $25.30 | $46.00 | $14.10–$32.56 | 45% |
| UF Health Leesburg Hospital Leesburg, FL · (352) 323-5762 HB VITAMIN D (25 HYDROXY) File of Mar 31, 2026 · source file | $29.15 | $53.00 | $23.85–$36.73 | 45% |
| The Villages Regional Hospital The Villages, FL · (352) 751-8000 HB VITAMIN D (25 HYDROXY) File of Mar 31, 2026 · source file | $29.15 | $53.00 | $23.85–$43.99 | 45% |
| Ucf Lake Nona Hospital Orlando, FL · (850) 523-2115 VITAMIN D 25OH W/WO FRAC File of Sep 1, 2026 · source file | $38.02 | $38.02 | $2.74–$34.22 | — |
| HCA Florida Lake Monroe Hospital Casselberry, FL · (407) 321-4500 VITAMIN D 25OH W/WO FRAC File of Sep 1, 2026 · source file | $40.00 | $40.00 | $2.88–$36.00 | — |
| AdventHealth Orlando Orlando, FL · (407) 303-1976 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY - ARUP File of Apr 1, 2026 · source file | $47.00 | $47.00 | — | — |
| Nemours Childrens Hospital, Florida Orlando, FL · (407) 567-4000 Vitamin D, 25+1 more line at this price File of Mar 10, 2026 · source file | $52.00 | $52.00 | — | — |
| AdventHealth Waterman Tavares, FL · (352) 253-3368 HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP File of Apr 1, 2026 · source file | $52.50 | $52.50 | — | — |
| Ucf Lake Nona Hospital Orlando, FL · (850) 523-2115 VITAMIN D3 25-OH File of Sep 1, 2026 · source file | $52.89 | $52.89 | $3.81–$47.60 | — |
| Oviedo Medical Center Orlando, FL · (615) 850-6055 VITAMIN D3 25-OH File of Sep 1, 2026 · source file | $52.97 | $52.97 | $3.81–$26.48 | — |
| Nemours Childrens Hospital, Florida Orlando, FL · (407) 567-4000 Vitamin D Referred+1 more line at this price File of Mar 10, 2026 · source file | $55.00 | $55.00 | — | — |
| HCA Florida Lake Monroe Hospital Casselberry, FL · (407) 321-4500 VITAMIN D3 25-OH File of Sep 1, 2026 · source file | $56.25 | $56.25 | $4.05–$50.63 | — |
| Oviedo Medical Center Orlando, FL · (615) 850-6055 VITAMIN D 25OH W/WO FRAC File of Sep 1, 2026 · source file | $56.50 | $56.50 | $4.07–$28.25 | — |
| UF Health Leesburg Hospital Leesburg, FL · (352) 323-5762 UF UC VITAMIN D HYDROXY File of Mar 31, 2026 · source file | $86.35 | $157.00 | $70.65–$108.80 | 45% |
| The Villages Regional Hospital The Villages, FL · (352) 751-8000 HB VITAMIN D HYDROXY File of Mar 31, 2026 · source file | $129.25 | $235.00 | $105.75–$195.05 | 45% |
| UF Health Leesburg Hospital Leesburg, FL · (352) 323-5762 HB VITAMIN D HYDROXY File of Mar 31, 2026 · source file | $129.25 | $235.00 | $14.10–$32.56 | 45% |
| AdventHealth Orlando Orlando, FL · (407) 303-1976 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY+1 more line at this price File of Apr 1, 2026 · source file | $194.00 | $194.00 | — | — |
| HCA Florida Osceola Hospital Orlando, FL · (407) 846-2266 VITAMIN D 25OH W/WO FRAC File of Sep 1, 2026 · source file | $201.00 | $201.00 | $14.47–$201.00 | — |
| Nemours Childrens Hospital, Florida Orlando, FL · (407) 567-4000 Vitamin D 25 Total+1 more line at this price File of Mar 10, 2026 · source file | $205.00 | $205.00 | — | — |
| AdventHealth Waterman Tavares, FL · (352) 253-3368 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY File of Apr 1, 2026 · source file | $529.22 | $529.22 | — | — |
Outpatient: lowest $11.95, median $25.30, highest $52.97 — a 4.4× difference within the same market.
As an inpatient, the same code is billed by 7 facilities here, median $24.00. Inpatient and outpatient prices are not comparable and are kept on separate pages: the inpatient charge covers admission, not the same purchase.
Cash or insurance?
At 2 of 6 hospitals that publish insurer rates for this code here, the cash price is below the highest rate a health plan has negotiated, and at none is it below every plan's rate. If you have insurance but have not met your deductible, you usually pay your plan's negotiated rate, which falls somewhere in the "Insurers pay" column. Paying cash can cost less, but a cash payment usually does not count toward your deductible. Ask the hospital for both numbers before you book.
What vitamin D test is, what the price covers and why prices differ: Vitamin D test explained.
4 rows held back from this comparison
They are priced below $1 or below a tenth of the national median for code 82306 ($100.88). Such a line is almost always a physician's fee billed separately, an assistant's share or a placeholder — a real charge, but not the price of the procedure itself.
| Hospital | Cash price | File |
|---|---|---|
| Orlando Health Central Ocoee, FLHb Vitamin D 25-Hydroxy-Ref | $8.80 | file |
| Orlando Health St Cloud Hospital Saint Cloud, FLHb Vitamin D 25-Hydroxy-Ref | $8.80 | file |
| South Lake Hospital Clermont, FLHb Vitamin D 25-Hydroxy-Ref | $8.80 | file |
| Orlando Health Orlando, FLHb Vitamin D 25-Hydroxy-Ref | $8.80 | file |