Vitamin D test cost in Orlando, FL
In Orlando-Kissimmee-Sanford, FL, 8 hospitals list a cash price for vitamin D blood test, 25-hydroxy (the standard vitamin D level test): from $24.00 to $52.50, with a median of $24.65. The lowest listed price is at South Lake Hospital and 3 other hospitals — $0.65 less than the median here. Across Florida, the median is $45.00 at 77 hospitals.
Cash prices at 8 facilities across 7 cities for code 82306, as an outpatient — the setting a self-pay patient normally buys. Collected Sep 23, 2026; the hospital files themselves were last updated between Mar 31, 2026 and Apr 1, 2026.
Where each hospital's price falls
Each dot is one hospital's cash price on a scale from the lowest to the highest in Orlando-Kissimmee-Sanford, FL.
Hospitals, cheapest first
| Hospital | Cash price | List price | Insurers pay | Off list | File date |
|---|---|---|---|---|---|
| South Lake Hospital lowest Clermont, FL · (352) 394-4071 Hb Vitamin D | $24.00 | $60.00 | — | 60% | Apr 1, 2026 source file |
| Orlando Health St Cloud Hospital lowest Saint Cloud, FL · (407) 892-2135 Hb Vitamin D | $24.00 | $60.00 | — | 60% | Apr 1, 2026 source file |
| Orlando Health lowest Orlando, FL · (321) 841-5111 Hb Vitamin D | $24.00 | $60.00 | — | 60% | Apr 1, 2026 source file |
| Orlando Health Central lowest Ocoee, FL · (407) 296-1000 Hb Vitamin D | $24.00 | $60.00 | — | 60% | Apr 1, 2026 source file |
| UF Health The Villages, FL · (352) 751-8000 UF HB 25-HYDROXY- D 2 AND D3, SERUM | $25.30 | $46.00 | $20.70–$38.18 | 45% | Mar 31, 2026 source file |
| UF Health Leesburg, FL · (352) 323-5762 UF HB 25-HYDROXY- D 2 AND D3, SERUM | $25.30 | $46.00 | $14.10–$32.56 | 45% | Mar 31, 2026 source file |
| UF Health The Villages, FL · (352) 751-8000 HB VITAMIN D (25 HYDROXY) | $29.15 | $53.00 | $23.85–$43.99 | 45% | Mar 31, 2026 source file |
| UF Health Leesburg, FL · (352) 323-5762 HB VITAMIN D (25 HYDROXY) | $29.15 | $53.00 | $23.85–$36.73 | 45% | Mar 31, 2026 source file |
| AdventHealth Orlando Orlando, FL · (407) 303-1976 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY - ARUP | $47.00 | $47.00 | — | — | Apr 1, 2026 source file |
| AdventHealth Waterman Tavares, FL · (352) 253-3368 HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $52.50 | $52.50 | — | — | Apr 1, 2026 source file |
| UF Health Leesburg, FL · (352) 323-5762 UF UC VITAMIN D HYDROXY | $86.35 | $157.00 | $70.65–$108.80 | 45% | Mar 31, 2026 source file |
| UF Health The Villages, FL · (352) 751-8000 HB VITAMIN D HYDROXY | $129.25 | $235.00 | $105.75–$195.05 | 45% | Mar 31, 2026 source file |
| UF Health Leesburg, FL · (352) 323-5762 HB VITAMIN D HYDROXY | $129.25 | $235.00 | $14.10–$32.56 | 45% | Mar 31, 2026 source file |
| AdventHealth Orlando Orlando, FL · (407) 303-1976 HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $194.00 | $194.00 | — | — | Apr 1, 2026 source file |
| AdventHealth Orlando Orlando, FL · (407) 303-1976 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $194.00 | $194.00 | — | — | Apr 1, 2026 source file |
| AdventHealth Waterman Tavares, FL · (352) 253-3368 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $529.22 | $529.22 | — | — | Apr 1, 2026 source file |
Outpatient: lowest $24.00, median $24.65, highest $52.50 — a 2.2× difference within the same market.
As an inpatient, the same code is billed by 6 facilities here, median $24.00. Inpatient and outpatient prices are not comparable and are kept on separate pages: the inpatient charge covers admission, not the same purchase.
Cash or insurance?
At 2 of 2 hospitals that publish insurer rates for this code here, the cash price is below the highest rate a health plan has negotiated, and at none is it below every plan's rate. If you have insurance but have not met your deductible, you usually pay your plan's negotiated rate, which falls somewhere in the "Insurers pay" column. Paying cash can cost less, but a cash payment usually does not count toward your deductible. Ask the hospital for both numbers before you book.
What it is
This blood test measures 25-hydroxy vitamin D, the form doctors use to judge whether the body has enough vitamin D. It is ordered to check for deficiency, for example with bone loss, certain digestive conditions or while taking supplements.
What the price covers
The price usually covers the vitamin D test only. The blood draw is often billed separately.
4 rows held back from this comparison
They are priced below $1 or below a tenth of the national median for code 82306 ($112.00). Such a line is almost always a physician's fee billed separately, an assistant's share or a placeholder — a real charge, but not the price of the procedure itself.
| Orlando Health Central Ocoee, FLHb Vitamin D 25-Hydroxy-Ref | $8.80 | file |
| South Lake Hospital Clermont, FLHb Vitamin D 25-Hydroxy-Ref | $8.80 | file |
| Orlando Health Orlando, FLHb Vitamin D 25-Hydroxy-Ref | $8.80 | file |
| Orlando Health St Cloud Hospital Saint Cloud, FLHb Vitamin D 25-Hydroxy-Ref | $8.80 | file |