Vitamin D test cost in Wisconsin
In Wisconsin, 80 hospitals publish a cash price for vitamin D blood test, 25-hydroxy (the standard vitamin D level test): from $26.07 to $445.55, with a median of $150.00. The lowest price is at Amery Hospital (East Amery). The highest price is 17.1× the lowest.
Outpatient cash prices, one line per hospital — its lowest listed price for code 82306. Prices for both sides of the body and inpatient prices are not mixed in. Collected Sep 24, 2026.
Lowest$26.07Amery Hospital
Median$150.00half pay less
Highest$445.55in Wisconsin
Lowest saves$123.93vs the median
Hospitals in Wisconsin, cheapest first
| Hospital | Cash price | List price | File date |
|---|---|---|---|
| Amery Hospital lowest East Amery · East Amery, WI Chemistry | $26.07 | $50.00 | — source file |
| Allina Health Systems River Falls · Minneapolis-St. Paul-Bloomington, MN-WI HCHG VITAMIN D TOTAL | $32.03 | $57.20 | Jun 15, 2026 source file |
| Bellin Memorial Health Green Bay · Green Bay, WI VITAMIN D; 25 HYDROXY | $85.66 | $185.00 | Jun 27, 2026 source file |
| Ascension NE Wisconsin Oshkosh · Oshkosh-Neenah, WI HC VITAMIN D 25 HYDROXY | $86.07 | $151.00 | — source file |
| Ascension NE Wisconsin - St Elizabeth Campus Appleton · Appleton, WI HC VITAMIN D 25 HYDROXY | $86.07 | $151.00 | — source file |
| UnityPoint Health - Meriter Madison · Madison, WI HC VITAMIN D, 25 HYDROXY | $88.88 | $111.10 | Jan 28, 2026 source file |
| Ascension SE Wisconsin Hospital Brookfield · Brookfield, WI HC VITAMIN D 25 HYDROXY | $93.28 | $176.00 | — source file |
| Ascension SE Wisconsin Hospital Milwaukee · Milwaukee-Waukesha, WI HC VITAMIN D 25 HYDROXY | $93.28 | $176.00 | — source file |
| Ascension St. Francis Hospital Milwaukee · Milwaukee-Waukesha, WI HC VITAMIN D 25 HYDROXY | $93.28 | $176.00 | — source file |
| Wheaton Franciscan Healthcare - Southeast Wisconsin Franklin · Milwaukee-Waukesha, WI HC VITAMIN D 25 HYDROXY | $93.28 | $176.00 | — source file |
| Columbia St. Mary's Glendale · Milwaukee-Waukesha, WI VIT D 25 HYDR SERUM OR PLASM | $104.94 | $198.00 | — source file |
| Westfields Hospital New Richmond · Minneapolis-St. Paul-Bloomington, MN-WI Chemistry | $109.41 | $208.00 | — source file |
| Children's Hospital of Wisconsin Neenah · Oshkosh-Neenah, WI HBC FV VITAMIN D 25 HYDROXY D2 D3 | $110.69 | $201.25 | Mar 24, 2026 source file |
| ThedaCare Regional Medical Center-Berlin Berlin · Berlin, WI VITAMIN D 25 HYDROXY D2/D3 | $120.68 | $215.50 | — source file |
| ThedaCare Regional Medical Center-Appleton Appleton · Appleton, WI VITAMIN D 25 HYDROXY | $120.68 | $215.50 | — source file |
| ThedaCare Regional Medical Center-Appleton Appleton · Appleton, WI VITAMIN D 25 HYDROXY | $120.68 | $215.50 | — source file |
| ThedaCare Regional Medical Center-Appleton Fond Du Lac · Fond du Lac, WI VITAMIN D 25 HYDROXY | $120.68 | $215.50 | — source file |
| ThedaCare Regional Medical Center-Appleton Oshkosh · Oshkosh-Neenah, WI VITAMIN D 25 HYDROXY | $120.68 | $215.50 | — source file |
| ThedaCare Regional Medical Center-Neenah Neenah · Oshkosh-Neenah, WI VITAMIN D 25 HYDROXY | $120.68 | $215.50 | — source file |
| ThedaCare Regional Medical Center-New London New London · New London, WI VITAMIN D 25 HYDROXY D2/D3 | $120.68 | $215.50 | — source file |
| ThedaCare Regional Medical Center-Shawano Shawano · Shawano, WI VITAMIN D 25 HYDROXY | $120.68 | $215.50 | — source file |
| ThedaCare Regional Medical Center-Waupaca Waupaca · Waupaca, WI VITAMIN D 25 HYDROXY | $120.68 | $215.50 | — source file |
| ThedaCare Regional Medical Center-Wild Rose Wild Rose · Wild Rose, WI VITAMIN D 25 HYDROXY | $120.68 | $215.50 | — source file |
| Ascension Calumet Hospital Chilton · Appleton, WI HC VITAMIN D 25 HYDROXY | $127.11 | $223.00 | — source file |
| Hudson Hospital Hudson · Minneapolis-St. Paul-Bloomington, MN-WI Chemistry | $129.47 | $287.00 | — source file |
| Memorial Hospital of Boscobel Boscobel · Platteville, WI VITAMIN D; 25 HYDROXY | $144.72 | $281.00 | Jun 27, 2026 source file |
| Aurora BayCare Medical Center Green Bay · Green Bay, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Lakeland Medical Center Elkhorn · Whitewater-Elkhorn, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Medical Center Bay Area Marinette · Marinette, WI-MI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Medical Center Burlington Burlington · Racine-Mount Pleasant, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Medical Center Fond du Lac Fond Du Lac · Fond du Lac, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Medical Center Grafton Grafton · Milwaukee-Waukesha, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Medical Center Kenosha Kenosha · Milwaukee-Waukesha, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Medical Center Manitowoc Two Rivers · Manitowoc, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Medical Center Mount Pleasant Mount Pleasant · Racine-Mount Pleasant, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Medical Center Oshkosh Oshkosh · Oshkosh-Neenah, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Medical Center Sheboygan County Sheboygan · Sheboygan, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Medical Center Summit Summit · Milwaukee-Waukesha, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Medical Center Washington County Hartford · Milwaukee-Waukesha, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Psychiatric Hospital Wauwatosa · Milwaukee-Waukesha, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora Sinai Medical Center of Aurora Health Care Metro Milwaukee · Milwaukee-Waukesha, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora St Lukes Medical Center of Aurora Health Care Metro Milwaukee · Milwaukee-Waukesha, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora St Lukes South Shore of Aurora Health Care Metro Cudahy · Milwaukee-Waukesha, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Aurora West Allis Medical Center West Allis · Milwaukee-Waukesha, WI VITAMIN D, 25-OH | $150.00 | $300.00 | Nov 21, 2025 source file |
| Gundersen Tri-County Hospital & Clinics Whitehall · Whitehall, WI VITAMIN D; 25 HYDROXY | $152.79 | $241.00 | Jun 27, 2026 source file |
| Gundersen St Josephs Hospital and Clinics Hillsboro · La Crosse-Onalaska, WI-MN VITAMIN D; 25 HYDROXY | $163.59 | $311.00 | Jun 27, 2026 source file |
| Marshfield Medical Center - Marshfield Marshfield · Wisconsin Rapids-Marshfield, WI VITAMIN D 25 HYDROXY-TECH | $169.10 | $178.00 | Feb 20, 2026 source file |
| Gundersen Moundview Hospital and Clinics Friendship · Friendship, WI VITAMIN D; 25 HYDROXY | $169.16 | $333.00 | Jun 27, 2026 source file |
| Aspirus Divine Savior Hospital Portage · Madison, WI 25 HYDROXY INCLUDES FRACTIONS IF PERFORMED | $178.10 | $274.00 | Apr 1, 2026 source file |
| Aspirus Eagle River Hospital Eagle River · Eagle River, WI 25 HYDROXY INCLUDES FRACTIONS IF PERFORMED | $178.10 | $274.00 | Apr 1, 2026 source file |
| Aspirus Langlade Hospital Antigo · Antigo, WI Fac Assay of Vitamin D | $178.10 | $274.00 | Apr 1, 2026 source file |
| Aspirus Medford Hospital Medford · Medford, WI 25 HYDROXY INCLUDES FRACTIONS IF PERFORMED | $178.10 | $274.00 | Apr 1, 2026 source file |
| Aspirus Merrill Hospital Merrill · Merrill, WI 25 HYDROXY INCLUDES FRACTIONS IF PERFORMED | $178.10 | $274.00 | Apr 1, 2026 source file |
| Aspirus Plover Hospital Stevens Point · Stevens Point-Plover, WI Fac Assay of Vitamin D | $178.10 | $274.00 | Apr 1, 2026 source file |
| Aspirus Rhinelander Hospital Rhinelander · Rhinelander, WI Fac Assay of Vitamin D | $178.10 | $274.00 | Apr 1, 2026 source file |
| Aspirus Stanley Hospital Stanley · Eau Claire, WI 25 HYDROXY INCLUDES FRACTIONS IF PERFORMED | $178.10 | $274.00 | Apr 1, 2026 source file |
| Aspirus Stevens Point Hospital Stevens Point · Stevens Point-Plover, WI Fac Assay of Vitamin D | $178.10 | $274.00 | Apr 1, 2026 source file |
| Aspirus Tomahawk Hospital Tomahawk · Tomahawk, WI Fac Assay of Vitamin D | $178.10 | $274.00 | Apr 1, 2026 source file |
| Aspirus Wausau Hospital Wausau · Wausau, WI 25 HYDROXY INCLUDES FRACTIONS IF PERFORMED | $178.10 | $274.00 | Apr 1, 2026 source file |
| Aspirus Wisconsin Rapids Hospital Wisconsin Rapids · Wisconsin Rapids-Marshfield, WI Fac Assay of Vitamin D | $178.10 | $274.00 | Apr 1, 2026 source file |
| Howard Young Medical Center Woodruff · Woodruff, WI 25 HYDROXY INCLUDES FRACTIONS IF PERFORMED | $178.10 | $274.00 | Apr 1, 2026 source file |
| St Nicholas Hospital of the Hospital Sisters of the Third Order of St Francis Sheboygan · Sheboygan, WI HC VITAMIN D 25 HYDROX-PRV 72 HR | $180.18 | $273.00 | — source file |
| St Vincent Hospital of the Hospital Sisters of the Third Order of St Francis Green Bay · Green Bay, WI HC VITAMIN D 25 HYDROX-PRV 72 HR | $180.18 | $273.00 | — source file |
| St Clare Memorial Hospital Oconto Falls · Green Bay, WI HC VITAMIN D 25 OH | $180.18 | $273.00 | — source file |
| St Marys Hospital Medical Center Green Bay · Green Bay, WI HC VITAMIN D 25 HYDROX-PRV 72 HR | $180.18 | $273.00 | — source file |
| Columbia St. Mary's Hospital Milwaukee Milwaukee · Milwaukee-Waukesha, WI HC VITAMIN D 25 HYDROXY | $184.97 | $349.00 | — source file |
| Columbia St. Mary's Hospital Ozaukee Mequon · Milwaukee-Waukesha, WI HC VITAMIN D 25 HYDROXY | $184.97 | $349.00 | — source file |
| Sacred Heart Rehabilitation Institute Mequon · Milwaukee-Waukesha, WI HC VITAMIN D 25 HYDROXY | $184.97 | $349.00 | — source file |
| Essentia Health St Marys Hospital-Superior Superior · Duluth, MN-WI VITAMIN D, 25 HYDROXY | $192.14 | $260.00 | Jan 1, 2026 source file |
| Marshfield Medical Center - Eau Claire Eau Claire · Eau Claire, WI VITAMIN D 25 HYDROXY-TECH | $267.90 | $282.00 | Feb 20, 2026 source file |
| Marshfield Medical Center - Ladysmith Ladysmith · Ladysmith, WI VITAMIN D 25 HYDROXY-TECH | $267.90 | $282.00 | Feb 20, 2026 source file |
| Marshfield Medical Center - Minocqua Minocqua · Minocqua, WI VITAMIN D 25 HYDROXY-TECH | $267.90 | $282.00 | Feb 20, 2026 source file |
| Marshfield Medical Center - Neillsville Neillsville · Neillsville, WI VITAMIN D 25 HYDROXY-TECH | $267.90 | $282.00 | Feb 20, 2026 source file |
| Marshfield Medical Center - Park Falls Park Falls · Park Falls, WI VITAMIN D 25 HYDROXY-TECH | $267.90 | $282.00 | Feb 20, 2026 source file |
| Marshfield Medical Center - Rice Lake Rice Lake · Rice Lake, WI VITAMIN D 25 HYDROXY-TECH | $267.90 | $282.00 | Feb 20, 2026 source file |
| Marshfield Medical Center - River Region at Stevens Point Stevens Point · Stevens Point-Plover, WI VITAMIN D 25 HYDROXY-TECH | $267.90 | $282.00 | Feb 20, 2026 source file |
| Marshfield Medical Center - Weston Weston · Wausau, WI VITAMIN D 25 HYDROXY-TECH | $267.90 | $282.00 | Feb 20, 2026 source file |
| AdventHealth Durand Durand · Durand, WI HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $346.40 | $346.40 | Apr 1, 2026 source file |
| Children's Hospital of Wisconsin Milwaukee · Milwaukee-Waukesha, WI HBC 25 OH VITAMIN D | $373.31 | $678.75 | Mar 23, 2026 source file |
| Marshfield Medical Center - Beaver Dam Beaver Dam · Beaver Dam, WI VITAMIN D 25 HYDROXY-TECH | $445.55 | $469.00 | Feb 20, 2026 source file |
By metro area
Each metro page compares every listing, including prices for both sides of the body.
| Metro area | Hospitals | Lowest | Median |
|---|---|---|---|
| Milwaukee-Waukesha, WI | 17 | $93.28 | $150.00 |
| Green Bay, WI | 5 | $85.66 | $180.18 |
| Oshkosh-Neenah, WI | 5 | $86.07 | $120.68 |
| Appleton, WI | 4 | $86.07 | $120.68 |
| Minneapolis-St. Paul-Bloomington, MN-WI | 3 | $32.03 | $109.41 |
| Stevens Point-Plover, WI | 3 | $178.10 | $178.10 |
| Eau Claire, WI | 2 | $178.10 | $223.00 |
| Fond du Lac, WI | 2 | $120.68 | $135.34 |
| Madison, WI | 2 | $88.88 | $133.49 |
| Racine-Mount Pleasant, WI | 2 | $150.00 | $150.00 |
| Sheboygan, WI | 2 | $150.00 | $165.09 |
| Wausau, WI | 2 | $178.10 | $223.00 |
| Wisconsin Rapids-Marshfield, WI | 2 | $169.10 | $173.60 |
| Antigo, WI | 1 | $178.10 | $178.10 |
| Berlin, WI | 1 | $120.68 | $120.68 |
| Brookfield, WI | 1 | $93.28 | $93.28 |
| Beaver Dam, WI | 1 | $445.55 | $445.55 |
| Durand, WI | 1 | $346.40 | $346.40 |
| Duluth, MN-WI | 1 | $192.14 | $192.14 |
| Eagle River, WI | 1 | $178.10 | $178.10 |
| East Amery, WI | 1 | $26.07 | $26.07 |
| Friendship, WI | 1 | $169.16 | $169.16 |
| Ladysmith, WI | 1 | $267.90 | $267.90 |
| La Crosse-Onalaska, WI-MN | 1 | $163.59 | $163.59 |
| Medford, WI | 1 | $178.10 | $178.10 |
| Merrill, WI | 1 | $178.10 | $178.10 |
| Minocqua, WI | 1 | $267.90 | $267.90 |
| Manitowoc, WI | 1 | $150.00 | $150.00 |
| Marinette, WI-MI | 1 | $150.00 | $150.00 |
| Neillsville, WI | 1 | $267.90 | $267.90 |
| New London, WI | 1 | $120.68 | $120.68 |
| Park Falls, WI | 1 | $267.90 | $267.90 |
| Platteville, WI | 1 | $144.72 | $144.72 |
| Rhinelander, WI | 1 | $178.10 | $178.10 |
| Rice Lake, WI | 1 | $267.90 | $267.90 |
| Shawano, WI | 1 | $120.68 | $120.68 |
| Tomahawk, WI | 1 | $178.10 | $178.10 |
| Waupaca, WI | 1 | $120.68 | $120.68 |
| Whitehall, WI | 1 | $152.79 | $152.79 |
| Wild Rose, WI | 1 | $120.68 | $120.68 |
| Woodruff, WI | 1 | $178.10 | $178.10 |
| Whitewater-Elkhorn, WI | 1 | $150.00 | $150.00 |
What it is
This blood test measures 25-hydroxy vitamin D, the form doctors use to judge whether the body has enough vitamin D. It is ordered to check for deficiency, for example with bone loss, certain digestive conditions or while taking supplements.
What the price covers
The price usually covers the vitamin D test only. The blood draw is often billed separately.
Vitamin D blood test, 25-hydroxy (the standard vitamin D level test) in other states
- Alabama$273.00
- Alaska$114.30
- Arizona$51.04
- Arkansas$77.00
- California$63.96
- Colorado$54.82
- Connecticut$100.65
- Delaware$267.63
- Florida$45.00
- Georgia$241.00
- Hawaii$81.00
- Idaho$32.81
- Illinois$183.60
- Indiana$134.75
- Iowa$96.80
- Kansas$222.95
- Kentucky$343.75
- Louisiana$68.42
- Maine$81.18
- Maryland$41.17
- Massachusetts$109.85
- Michigan$91.00
- Minnesota$96.80
- Mississippi$48.48
- Missouri$129.63
- Montana$88.35
- Nebraska$89.09
- Nevada$110.25
- New Hampshire$143.98
- New Jersey$34.04
- New Mexico$115.20
- New York$89.00
- North Carolina$168.00
- North Dakota$76.38
- Ohio$76.95
- Oklahoma$131.17
- Oregon$88.50
- Pennsylvania$149.40
- Puerto Rico$47.70
- Rhode Island$145.00
- South Carolina$151.45
- South Dakota$96.80
- Tennessee$52.05
- Texas$49.20
- Utah$84.70
- Vermont$212.00
- Virginia$118.40
- Washington$137.20
- West Virginia$71.50
- Wyoming$57.14
Median cash price across the state's hospitals.