Vitamin D test cost in Pittsburgh, PA
In Pittsburgh, PA, 14 hospitals list a cash price for Vitamin D blood test, 25-hydroxy (the standard vitamin D level test): from $13.00 to $532.20, with a median of $207.30. The lowest listed price is at Uniontown Hospital — $194.30 less than the median here. Across Pennsylvania, the median is $149.40 at 65 hospitals.
Cash prices at 14 facilities across 9 cities for code 82306, as an outpatient — the setting a self-pay patient normally buys. Collected Sep 23, 2026; the hospital files themselves were last updated between Mar 6, 2026 and Apr 14, 2026.
Where each hospital's price falls
Each dot is one hospital's cash price on a scale from the lowest to the highest in Pittsburgh, PA.
Hospitals, cheapest first
| Hospital | Cash price | List price | Insurers pay | Off list | File date |
|---|---|---|---|---|---|
| Uniontown Hospital lowest Uniontown, PA VITAMIN D 25 HYDROXY | $13.00 | $26.00 | $26.00–$171.55 | 50% | — source file |
| West Penn Allegheny Health System INC Wexford, PA CHG 25 HYDROXY INCLUDES FRACTIONS IF PERFORMED | $22.88 | $88.00 | $14.34–$89.98 | 74% | Apr 14, 2026 source file |
| Uniontown Hospital Uniontown, PA VITAMIN D-3 LEVEL | $71.50 | $143.00 | $27.97–$171.55 | 50% | — source file |
| UPMC Washington Washington, PA VITAMIN D-25 DIHYDROXY | $94.29 | $134.70 | $20.72–$96.98 | 30% | — source file |
| UPMC Washington Washington, PA VITAMIN D (25 HYDROXYO | $94.29 | $134.70 | $20.72–$96.98 | 30% | — source file |
| UPMC Jameson New Castle, PA VITAMIN D/25-HYDROXY | $100.20 | $167.00 | $29.26–$174.22 | 40% | — source file |
| UPMC Jameson New Castle, PA 25-OH VITAMIN D3 P3433 | $100.20 | $167.00 | $29.26–$174.22 | 40% | — source file |
| UPMC Jameson New Castle, PA CARD IQ VIT D 25HYDRX LC/MS/MS | $100.20 | $167.00 | $29.26–$174.22 | 40% | — source file |
| UPMC Jameson New Castle, PA VITAMIN D IMMUNOASSAY | $100.20 | $167.00 | $29.26–$174.22 | 40% | — source file |
| UPMC Presbyterian Pittsburgh, PA VITAMIN D/25-HYDROXY | $169.20 | $282.00 | $29.26–$257.13 | 40% | Mar 6, 2026 source file |
| UPMC Presbyterian Pittsburgh, PA 25-HYDROXY-VITAMIN-D | $169.20 | $282.00 | $29.26–$257.13 | 40% | Mar 6, 2026 source file |
| UPMC Presbyterian Pittsburgh, PA CARD IQ VIT D 25HYDRX LC/MS/MS | $169.20 | $282.00 | $29.26–$257.13 | 40% | Mar 6, 2026 source file |
| UPMC Presbyterian Pittsburgh, PA VITAMIN D IMMUNOASSAY | $169.20 | $282.00 | $29.26–$257.13 | 40% | Mar 6, 2026 source file |
| UPMC Presbyterian Pittsburgh, PA 25-OH VITAMIN D3 P3433 | $169.20 | $282.00 | $29.26–$257.13 | 40% | Mar 6, 2026 source file |
| West Penn Allegheny Health System INC Wexford, PA HC VITAMIN D 25 HYDROXY | $187.22 | $253.00 | $14.34–$89.98 | 26% | Apr 14, 2026 source file |
| UPMC Childrens Hospital of Pittsburgh Pittsburgh, PA VITAMIN D/25-HYDROXY | $194.40 | $324.00 | $26.38–$291.60 | 40% | Mar 6, 2026 source file |
| UPMC Childrens Hospital of Pittsburgh Pittsburgh, PA QUESTASSURE D INF 25-HYDROX D | $194.40 | $324.00 | $26.38–$291.60 | 40% | Mar 6, 2026 source file |
| UPMC Childrens Hospital of Pittsburgh Pittsburgh, PA CARD IQ VIT D 25HYDRX LC/MS/MS | $194.40 | $324.00 | $26.38–$291.60 | 40% | Mar 6, 2026 source file |
| UPMC Childrens Hospital of Pittsburgh Pittsburgh, PA 25-OH VITAMIN D3 P3433 | $194.40 | $324.00 | $26.38–$291.60 | 40% | Mar 6, 2026 source file |
| UPMC Childrens Hospital of Pittsburgh Pittsburgh, PA 25-HYDROXY-VITAMIN-D | $194.40 | $324.00 | $26.38–$291.60 | 40% | Mar 6, 2026 source file |
| UPMC Magee-Womens Hospital Pittsburgh, PA HC VITAMIN D 25 HYDROXY INC FRACTION | $205.80 | $343.00 | $27.70–$315.56 | 40% | — source file |
| Highlands Hospital Connellsville, PA VITAMIN D,25-HYDROXY TOTAL,D2&D3 | $208.80 | $348.00 | $117.28–$271.44 | 40% | — source file |
| Monogahela Valley Hospital Monongahela, PA 25 HYDROXY VIT D | $208.80 | $348.00 | $93.61–$426.47 | 40% | — source file |
| Highlands Hospital Connellsville, PA VITAMIN D LEVEL | $208.80 | $348.00 | $117.28–$271.44 | 40% | — source file |
| UPMC Mercy Pittsburgh, PA VITAMIN D IMMUNOASSAY | $223.80 | $373.00 | $24.67–$335.70 | 40% | — source file |
| UPMC Mercy Pittsburgh, PA VITAMIN D/25-HYDROXY | $223.80 | $373.00 | $24.67–$335.70 | 40% | — source file |
| UPMC Mercy Pittsburgh, PA 25-OH VITAMIN D3 P3433 | $223.80 | $373.00 | $24.67–$335.70 | 40% | — source file |
| UPMC Mercy Pittsburgh, PA 25-HYDROXY-VITAMIN-D | $223.80 | $373.00 | $24.67–$335.70 | 40% | — source file |
| UPMC Mercy Pittsburgh, PA CARD IQ VIT D 25HYDRX LC/MS/MS | $223.80 | $373.00 | $24.67–$335.70 | 40% | — source file |
| UPMC St Margaret Pittsburgh, PA VITAMIN D IMMUNOASSAY | $250.80 | $418.00 | $25.56–$355.30 | 40% | — source file |
| UPMC St Margaret Pittsburgh, PA VITAMIN D/25-HYDROXY | $250.80 | $418.00 | $25.56–$355.30 | 40% | — source file |
| UPMC St Margaret Pittsburgh, PA 25-OH VITAMIN D3 P3433 | $250.80 | $418.00 | $25.56–$355.30 | 40% | — source file |
| UPMC St Margaret Pittsburgh, PA CARD IQ VIT D 25HYDRX LC/MS/MS | $250.80 | $418.00 | $25.56–$355.30 | 40% | — source file |
| UPMC St Margaret Pittsburgh, PA 25-HYDROXY-VITAMIN-D | $250.80 | $418.00 | $25.56–$355.30 | 40% | — source file |
| UPMC McKeesport McKeesport, PA VITAMIN D/25-HYDROXY | $265.20 | $442.00 | $27.44–$375.70 | 40% | — source file |
| UPMC McKeesport McKeesport, PA CARD IQ VIT D 25HYDRX LC/MS/MS | $265.20 | $442.00 | $27.44–$375.70 | 40% | — source file |
| UPMC McKeesport McKeesport, PA 25-OH VITAMIN D3 P3433 | $265.20 | $442.00 | $27.44–$375.70 | 40% | — source file |
| UPMC East Monroeville, PA 25-OH VITAMIN D3 P3433 | $441.60 | $736.00 | $29.26–$588.80 | 40% | — source file |
| UPMC East Monroeville, PA VITAMIN D/25-HYDROXY | $441.60 | $736.00 | $29.26–$588.80 | 40% | — source file |
| UPMC Passavant - McCandless Pittsburgh, PA HC VITAMIN D 25 HYDROXY INC FRACTION | $532.20 | $887.00 | $27.51–$753.95 | 40% | — source file |
Outpatient: lowest $13.00, median $207.30, highest $532.20 — a 40.9× difference within the same market.
As an inpatient, the same code is billed by 13 facilities here, median $208.80. Inpatient and outpatient prices are not comparable and are kept on separate pages: the inpatient charge covers admission, not the same purchase.
Cash or insurance?
At 14 of 14 hospitals that publish insurer rates for this code here, the cash price is below the highest rate a health plan has negotiated, and at 1 it is below every plan's rate. If you have insurance but have not met your deductible, you usually pay your plan's negotiated rate, which falls somewhere in the "Insurers pay" column. Paying cash can cost less, but a cash payment usually does not count toward your deductible. Ask the hospital for both numbers before you book.
What it is
This blood test measures 25-hydroxy vitamin D, the form doctors use to judge whether the body has enough vitamin D. It is ordered to check for deficiency, for example with bone loss, certain digestive conditions or while taking supplements.
What the price covers
The price usually covers the vitamin D test only. The blood draw is often billed separately.
2 rows held back from this comparison
They are priced below $1 or below a tenth of the national median for code 82306 ($112.00). Such a line is almost always a physician's fee billed separately, an assistant's share or a placeholder — a real charge, but not the price of the procedure itself.
| UPMC Magee-Womens Hospital Pittsburgh, PAHC NEO ERGOCALCIFEROL 10MCG/ML O/S | $5.40 | file |
| UPMC Passavant - McCandless Pittsburgh, PAHC NEO ERGOCALCIFEROL 10MCG/ML O/S | $5.40 | file |