Vitamin D test cost in Chicago, IL
In Chicago-Naperville-Elgin, IL-IN, 63 hospitals list a cash price for vitamin D blood test, 25-hydroxy (the standard vitamin D level test): from $11.40 to $558.52, with a median of $191.10. The lowest listed price is at Gottlieb Memorial Hospital and 1 other hospital — $179.70 less than the median here. Across Illinois, the median is $182.20 at 114 hospitals.
- The median here, $191.10, is 66% above the US median of $114.80.
- That is 6.5× Medicare's 2026 national rate for the same service ($29.60).
- 12 of 63 hospitals list a cash price equal to their full list price, with no self-pay discount.
Cash prices at 63 facilities across 44 cities for code 82306, as an outpatient — the setting a self-pay patient normally buys. Collected Nov 10, 2026; the hospital files themselves were last updated between Jan 1, 2025 and Nov 6, 2026.
Lowest$11.40Gottlieb Memorial Hospital + 1 more
Median$191.10half of hospitals charge less
Highest$558.52in this market
Difference49.0×highest vs lowest
Where each hospital's price falls
Each dot is one hospital's cash price on a scale from the lowest to the highest in Chicago-Naperville-Elgin, IL-IN.
median
Lowest $11.40Highest $558.52
Hospitals, cheapest first
| Hospital | Cash price | List price | Insurers pay | Off list |
|---|---|---|---|---|
| Gottlieb Memorial Hospital lowest Melrose Park, IL · (708) 681-3200 Vit D Hydroxy Incl Fracts (Wvitd) File of Mar 31, 2026 · source file | $11.40 | $60.00 | $25.56–$50.28 | 81% |
| MacNeal Hospital lowest Berwyn, IL · (708) 783-9100 Vit D Hydroxy Incl Fracts (Wvitd) File of Mar 31, 2026 · source file | $11.40 | $60.00 | $11.40–$83.10 | 81% |
| Loyola University Medical Center Maywood, IL · (708) 216-9000 Vit D Hydroxy Incl Fracts (Wvitd) File of Mar 31, 2026 · source file | $13.80 | $60.00 | $32.99 | 77% |
| MacNeal Hospital Berwyn, IL · (708) 783-9100 Vitamin D 25-Hydroxy (Vitd) File of Mar 31, 2026 · source file | $24.32 | $128.00 | $24.32–$177.28 | 81% |
| Gottlieb Memorial Hospital Melrose Park, IL · (708) 681-3200 Vitamin D 25-Hydroxy (Vitd) File of Mar 31, 2026 · source file | $24.32 | $128.00 | $54.53–$107.26 | 81% |
| Uchicago Medicine AdventHealth Hinsdale Hinsdale, IL · (630) 856-9000 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY+1 more line at this price File of Apr 1, 2026 · source file | $26.00 | $104.00 | — | 75% |
| Uchicago Medicine AdventHealth Glenoaks Glendale Heights, IL · (630) 545-8000 HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP+1 more line at this price File of Apr 1, 2026 · source file | $26.00 | $104.00 | — | 75% |
| Uchicago Medicine AdventHealth Bolingbrook Bolingbrook, IL · (630) 312-5000 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY+1 more line at this price File of Apr 1, 2026 · source file | $26.00 | $104.00 | — | 75% |
| Uchicago Medicine AdventHealth La Grange La Grange, IL · (708) 352-1200 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY+1 more line at this price File of Apr 1, 2026 · source file | $26.00 | $104.00 | — | 75% |
| Roseland Community Hospital Chicago, IL · (773) 995-3000 Vitamin D-3 level File of Jul 1, 2026 · source file | $28.66 | $78.00 | $27.53–$85.80 | 63% |
| Saint Joseph Medical Center - Joliet Joliet, IL · (815) 725-7133 VITAMIN D 25 OH (D2,D3) GROUP+1 more line at this price File of Sep 1, 2026 · source file | $29.00 | $733.00 | $17.76–$733.00 | 96% |
| Mercy Medical Center Aurora, IL · (630) 859-2222 VITAMIN D 25 HYDROXY+1 more line at this price File of Sep 1, 2026 · source file | $29.00 | $721.00 | $17.76–$721.00 | 96% |
| Saint Francis Hospital Evanston, IL · (847) 316-4000 HC ASSAY VIT D, 25-HYDROXY W FRACTIONS File of Sep 1, 2026 · source file | $29.00 | $189.00 | $17.76–$189.00 | 85% |
| Loyola University Medical Center Maywood, IL · (708) 216-9000 Vitamin D 25-Hydroxy (Vitd) File of Mar 31, 2026 · source file | $29.44 | $128.00 | $56.19–$134.91 | 77% |
| John H. Stroger, Jr. Hospital Chicago, IL · (312) 864-6000 VITAMIN D-3 25-OH File of Apr 1, 2026 · source file | $51.80 | $74.00 | $8.31–$59.20 | 30% |
| Franciscan Health Dyer Dyer, IN · (219) 865-2141 Vitamin D; 25 Hydroxy File of Jan 1, 2026 · source file | $52.11 | $193.00 | $26.49–$144.75 | 73% |
| Franciscan Health Munster Munster, IN · (219) 922-4200 Vitamin D; 25 Hydroxy File of Jan 1, 2026 · source file | $56.23 | $264.00 | $23.38–$198.00 | 79% |
| MacNeal Hospital Berwyn, IL · (708) 783-9100 Vit D 25hydroxy,Includes Fract (Zvitd) File of Mar 31, 2026 · source file | $56.43 | $297.00 | $56.43–$411.35 | 81% |
| Gottlieb Memorial Hospital Melrose Park, IL · (708) 681-3200 Vit D 25hydroxy,Includes Fract (Zvitd) File of Mar 31, 2026 · source file | $56.43 | $297.00 | $126.52–$248.89 | 81% |
| Presence Saint Joseph Hospital - Chicago Chicago, IL · (773) 665-3000 HC VITAMIN D (25-OH) TOTAL+1 more line at this price File of Jan 1, 2026 · source file | $62.37 | $189.00 | $29.60–$122.59 | 67% |
| Franciscan Health Rensselaer Rensselaer, IN · (219) 866-5141 Vitamin D; 25 Hydroxy File of Jan 1, 2026 · source file | $64.44 | $180.00 | $26.49–$144.00 | 64% |
| Loyola University Medical Center Maywood, IL · (708) 216-9000 Vit D 25hydroxy,Includes Fract (Zvitd) File of Mar 31, 2026 · source file | $68.31 | $297.00 | $32.99 | 77% |
| Northwest Health - Porter Valparaiso, IN · (219) 983-8300 Vitamin D 25 hydroxy File of Apr 1, 2026 · source file | $77.76 | $288.00 | $26.11–$241.92 | 73% |
| Northwest Community Hospital Arlington Heights, IL · (847) 618-1000 Hb Vitamin D 25 Hydroxy File of Apr 1, 2026 · source file | $90.00 | $90.00 | — | — |
| Loretto Hospital Chicago, IL · (773) 626-4300 VITAMIN D 1,25 DIHYDROXY File of Nov 5, 2026 · source file | $93.32 | $155.53 | $93.32–$301.00 | 40% |
| Humboldt Park Health Chicago, IL · (773) 292-8200 Vitamin D-3 level File of Sep 29, 2026 · source file | $94.37 | $337.05 | $111.23–$353.90 | 72% |
| Morris Hospital Morris, IL · (815) 942-2932 Vitamin D-3 level File of Oct 27, 2026 · source file | $99.37 | $413.00 | $178.42–$425.39 | 76% |
| Franciscan Health Crown Point Crown Point, IN · (219) 757-6100 Vitamin D; 25 Hydroxy File of Jan 1, 2026 · source file | $99.85 | $387.00 | $387.00 | 74% |
| Community First Medical Center Chicago, IL · (773) 282-7000 Vitamin D-3 level File of Apr 1, 2026 · source file | $103.75 | $207.50 | $27.86–$207.50 | 50% |
| NorthShore University HealthSystems Glenbrook Hospital Glenview, IL Vitamin D25 Hydroxy*+1 more line at this price File of Jan 1, 2025 · source file | $125.45 | $193.00 | $26.06–$105.38 | 35% |
| NorthShore University HealthSystems Skokie Hospital Skokie, IL Vitamin D25 Hydroxy*+1 more line at this price File of Jan 1, 2025 · source file | $125.45 | $193.00 | $26.06–$105.38 | 35% |
| Resilience Healthcare West Suburban Medical Center Oak Park, IL · (708) 383-6200 Vitamin D 25 Hydroxy Level+1 more line at this price File of Mar 17, 2025 · source file | $149.70 | $440.28 | $23.76–$286.18 | 66% |
| Loretto Hospital Chicago, IL · (773) 626-4300 VITAMIN D, 25-HYDROXY File of Nov 5, 2026 · source file | $180.60 | $301.00 | $93.32–$301.00 | 40% |
| Louis A Weiss Memorial Hospital Chicago, IL · (773) 878-8700 25-Hydroxyvitamin D LCMS D2+D3 SO+1 more line at this price File of Mar 17, 2025 · source file | $189.92 | $558.59 | $23.76–$363.08 | 66% |
| Northwestern Medicine Delnor Hospital Geneva, IL · (630) 208-3000 HB Ref Vitamin D 25-Hydroxy (D2,D3), Lc-Ms-Ms (Lc)+7 more lines at this price File of Apr 1, 2026 · source file | $191.10 | $273.00 | $40.68–$273.00 | 30% |
| Northwestern Lake Forest Hospital Lake Forest, IL · (847) 234-5600 HB Ref Vitamin D 25-Hydroxy (D2,D3) (Q)+7 more lines at this price File of Apr 1, 2026 · source file | $191.10 | $273.00 | $45.32–$273.00 | 30% |
| Palos Community Hospital Palos Heights, IL · (708) 923-4000 HB Ref 25-Hydroxyvitamin D (Mayo)+7 more lines at this price File of Apr 1, 2026 · source file | $191.10 | $273.00 | $43.68–$273.00 | 30% |
| Northwestern Medicine Central DuPage Hospital Winfield, IL · (630) 682-1600 HB Ref 25-Hydroxyvitamin D (Mayo)+7 more lines at this price File of Apr 1, 2026 · source file | $191.10 | $273.00 | $18.84–$273.00 | 30% |
| Northwestern Medicine McHenry Hospital McHenry, IL · (815) 344-5000 HB Ref 25-Hydroxyvitamin D (Mayo)+7 more lines at this price File of Apr 1, 2026 · source file | $191.10 | $273.00 | $52.69–$273.00 | 30% |
| Northwestern Medicine Valley West Hospital Sandwich, IL · (815) 786-8484 HB Ref 25-Hydroxyvitamin D (Mayo)+7 more lines at this price File of Apr 1, 2026 · source file | $191.10 | $273.00 | $51.87–$273.00 | 30% |
| Northwestern Memorial Hospital Chicago, IL · (312) 926-2000 HB Ref 25-Hydroxyvitamin D (Mayo)+7 more lines at this price File of Apr 1, 2026 · source file | $191.10 | $273.00 | $52.96–$273.00 | 30% |
| Northwestern Medicine Kishwaukee Hospital DeKalb, IL · (815) 756-1521 HB Ref 25-Hydroxyvitamin D (Mayo)+7 more lines at this price File of Apr 1, 2026 · source file | $191.10 | $273.00 | $49.96–$273.00 | 30% |
| La Rabida Children's Hospital Chicago, IL · (773) 363-6700 VITAMIN D 25-OH File of Apr 1, 2026 · source file | $191.25 | $191.25 | — | — |
| Endeavor Health Highland Park Hospital Highland Park, IL HB VITAMIN D+1 more line at this price File of Apr 1, 2026 · source file | $193.00 | $193.00 | — | — |
| Northshore University Healthsystem - Evanston Hospital Evanston, IL · (847) 570-2000 HB VITAMIN D+1 more line at this price File of Apr 1, 2026 · source file | $193.00 | $193.00 | — | — |
| Swedish Covenant Health Chicago, IL · (773) 878-8200 HB VITAMIN D25 HYDROXY*+1 more line at this price File of Apr 1, 2026 · source file | $193.00 | $193.00 | — | — |
| Advocate Lutheran General Hospital Park Ridge, IL · (847) 723-2210 VITAMIN D, 25-OH File of Nov 3, 2026 · source file | $222.50 | $445.00 | $26.31–$435.64 | 50% |
| Advocate Good Samaritan Hospital Downers Grove, IL · (630) 275-5900 VITAMIN D, 25-OH File of Nov 3, 2026 · source file | $222.50 | $445.00 | $26.31–$439.77 | 50% |
| Advocate Childrens Hospital Park Ridge Park Ridge, IL VITAMIN D, 25-OH File of Nov 6, 2026 · source file | $222.50 | $445.00 | $26.31–$443.90 | 50% |
| Advocate Condell Medical Center Libertyville, IL · (847) 362-2900 VITAMIN D, 25-OH File of Nov 3, 2026 · source file | $222.50 | $445.00 | $26.31–$356.00 | 50% |
| Advocate Trinity Hospital Chicago, IL · (773) 967-5002 VITAMIN D, 25-OH File of Nov 3, 2026 · source file | $222.50 | $445.00 | $26.31–$439.77 | 50% |
| Advocate Childrens Hospital Oak Lawn Oak Lawn, IL VITAMIN D, 25-OH File of Nov 3, 2026 · source file | $222.50 | $445.00 | $26.31–$437.54 | 50% |
| Advocate Christ Medical Center Oak Lawn, IL · (708) 684-8000 VITAMIN D, 25-OH File of Nov 3, 2026 · source file | $222.50 | $445.00 | $26.31–$429.60 | 50% |
| Advocate Good Shepherd Hospital Barrington, IL · (847) 381-9600 VITAMIN D, 25-OH File of Nov 3, 2026 · source file | $222.50 | $445.00 | $26.31–$442.63 | 50% |
| Advocate South Suburban Hospital Hazel Crest, IL VITAMIN D, 25-OH File of Nov 3, 2026 · source file | $222.50 | $445.00 | $26.31–$441.35 | 50% |
| Advocate Illinois Masonic Medical Center Chicago, IL · (773) 975-1600 VITAMIN D, 25-OH File of Nov 3, 2026 · source file | $222.50 | $445.00 | $26.31–$442.63 | 50% |
| Advocate Sherman Hospital Elgin, IL · (847) 742-9800 VITAMIN D, 25-OH File of Nov 3, 2026 · source file | $222.50 | $445.00 | $26.31–$400.50 | 50% |
| Shriners Childrens - Chicago Chicago, IL · (773) 622-5400 VITAMIN D-3 LEVEL File of Mar 19, 2026 · source file | $253.90 | $253.90 | $26.33–$53.55 | — |
| Mercyhealth Crystal Lake Hospital & Medical Center Crystal Lake, IL · (792) 220-5500 CHG 25 HYDROXY INCLUDES FRACTIONS IF PERFORMED File of Mar 1, 2026 · source file | $282.80 | $377.06 | — | 25% |
| The University of Chicago Medical Center Chicago, IL · (773) 702-9785 Hc Vitamin D; 25 Hydroxy, Includes Fraction(S), If Performed-Laf File of Apr 1, 2026 · source file | $283.00 | $283.00 | — | — |
| Ingalls Memorial Hospital Harvey, IL · (708) 333-2300 Hc Vitamin D; 25 Hydroxy, Includes Fraction(S), If Performed File of Apr 1, 2026 · source file | $293.00 | $293.00 | — | — |
| Uchicago Medicine Northwest Indiana Crown Point, IN · (312) 755-4706 HC VITAMIN D; 25 HYDROXY, INCLUDES FRACTION(S), IF PERFORMED File of Apr 1, 2026 · source file | $293.00 | $293.00 | — | — |
| Jackson Park Hospital Foundation Chicago, IL · (773) 947-7500 VITAMIN D 25 HYDROXY File of Dec 26, 2025 · source file | $301.80 | $377.25 | — | 20% |
| Mercyhealth Hospital & Medical Center - Harvard Harvard, IL · (815) 943-5431 CHG 25 HYDROXY INCLUDES FRACTIONS IF PERFORMED File of Mar 1, 2026 · source file | $320.05 | $426.73 | — | 25% |
| Ann & Robert H. Lurie Children's Hospital of Chicago Chicago, IL · (312) 227-4000 H 25-OH VITAMIN D - TOTAL CALCIDIOL+1 more line at this price File of Sep 1, 2026 · source file | $364.00 | $520.00 | $84.00–$494.00 | 30% |
| Elmhurst Memorial Hospital Elmhurst, IL · (331) 221-1000 HC VITAMIN D 25 HYDROXY File of Apr 1, 2026 · source file | $404.00 | $404.00 | — | — |
| Edward Hospital Naperville, IL · (630) 527-3000 HC VITAMIN D 25 HYDROXY File of Apr 1, 2026 · source file | $404.00 | $404.00 | — | — |
| Methodist Hospital-Southlake Merrillville, IN · (219) 886-4000 Vitamin D 25 Hydroxy File of Aug 18, 2026 · source file | $412.30 | $589.00 | $29.60–$524.21 | 30% |
| The Methodist Hospitals Gary, IN · (219) 886-4000 Vitamin D 25 Hydroxy File of Aug 18, 2026 · source file | $412.30 | $589.00 | $29.60–$524.21 | 30% |
| The University of Chicago Medical Center Chicago, IL · (773) 702-9785 Hc Vitamin D; 25 Hydroxy, Includes Fraction(S), If Performed File of Apr 1, 2026 · source file | $469.00 | $469.00 | — | — |
| Vista Medical Center East Waukegan, IL · (847) 360-3000 VITAMIN D 25OH +2 more lines at this price File of Mar 31, 2025 · source file | $558.52 | $558.52 | $29.60–$78.75 | — |
Hospital The hospital that published the price, its city and the line exactly as written in its price file.
Cash price The hospital's own price for a patient paying without insurance (“discounted cash” in its price file). Hospitals must publish it under federal law, 45 CFR 180.50. Call to confirm it before booking. More
List price The hospital's full chargemaster price (“gross charge”) before any discount. Almost nobody pays it; the gap to the cash price shows what the self-pay discount is worth. More
Insurers pay The lowest and highest rates this hospital has agreed with insurance plans for the same item, from its price file. If the cash price is below what your plan pays and you have not met your deductible, paying cash can cost you less. More
Off list How much lower the cash price is than the list price.
No cash discount This line's cash price equals the hospital's full list price. Many hospitals still reduce bills for uninsured patients: ask the billing office for its self-pay discount in writing. More
Outpatient: lowest $11.40, median $191.10, highest $558.52 — a 49.0× difference within the same market.
As an inpatient, the same code is billed by 41 facilities here, median $193.00. Inpatient and outpatient prices are not comparable and are kept on separate pages: the inpatient charge covers admission, not the same purchase.
Cash or insurance?
At 42 of 46 hospitals that publish insurer rates for this code here, the cash price is below the highest rate a health plan has negotiated, and at 5 it is below every plan's rate. If you have insurance but have not met your deductible, you usually pay your plan's negotiated rate, which falls somewhere in the "Insurers pay" column. Paying cash can cost less, but a cash payment usually does not count toward your deductible. Ask the hospital for both numbers before you book.
What vitamin D test is, what the price covers and why prices differ: Vitamin D test explained.