Vitamin D test cost in Charlotte, NC — inpatient
In Charlotte-Concord-Gastonia, NC-SC, 16 hospitals list a cash price for Vitamin D blood test, 25-hydroxy (the standard vitamin D level test) as an inpatient: from $153.75 to $1,014.00, with a median of $224.53. The lowest listed price is at Amisub of South Carolina, Inc. and 1 other hospital — $70.78 less than the median here. Across North Carolina, the median is $168.00 at 37 hospitals.
Cash prices at 16 facilities across 13 cities for code 82306, as an inpatient (admitted to hospital) . Collected Sep 23, 2026; the hospital files themselves were last updated between Dec 1, 2025 and Sep 1, 2026.
Where each hospital's price falls
Each dot is one hospital's cash price on a scale from the lowest to the highest in Charlotte-Concord-Gastonia, NC-SC.
Hospitals, cheapest first
| Hospital | Cash price | List price | Insurers pay | Off list | File date |
|---|---|---|---|---|---|
| Amisub of South Carolina, Inc. lowest Rock Hill, SC VITAMIN D/25 HYDROXY/3 | $153.75 | $205.00 | $1.00–$243.12 | 25% | Sep 1, 2026 source file |
| Amisub of South Carolina, Inc. lowest Fort Mill, SC VITAMIN D/25 HYDROXY/3 | $153.75 | $205.00 | $1.00–$243.12 | 25% | Apr 15, 2026 source file |
| The Charlotte Mecklenburg Hospital Authority Charlotte, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | $136.06–$413.13 | 50% | Dec 5, 2025 source file |
| Carolinas Medical Center Charlotte, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | $136.06–$404.15 | 50% | Dec 5, 2025 source file |
| The Charlotte Mecklenburg Hospital Authority Charlotte, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | $136.06–$413.13 | 50% | Dec 5, 2025 source file |
| Atrium Health Hospitals Inc Lincolnton, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | $128.74–$426.60 | 50% | Dec 1, 2025 source file |
| Carolinas Medical Center Charlotte, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | $136.06–$413.13 | 50% | Dec 4, 2025 source file |
| Carolinas Medical Center Charlotte, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | $139.21–$395.16 | 50% | Dec 4, 2025 source file |
| Atrium Health Hospitals Inc Wadesboro, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | $136.06–$426.60 | 50% | Dec 1, 2025 source file |
| The Charlotte Mecklenburg Hospital Authority Concord, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | $136.06–$426.60 | 50% | Dec 1, 2025 source file |
| Carolinas Medical Center Charlotte, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | $136.06–$404.15 | 50% | Dec 4, 2025 source file |
| The Charlotte Mecklenburg Hospital Authority Monroe, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | $136.06–$426.60 | 50% | Dec 4, 2025 source file |
| Carolinas Rehabilitation Charlotte, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | $150.43–$413.13 | 50% | Dec 5, 2025 source file |
| Carolinas Rehabilitation Belmont, NC HC ASSAY OF VITAMIN D 25 | $224.53 | $449.05 | $150.43–$413.13 | 50% | Dec 5, 2025 source file |
| DUKE HEALTH LAKE NORMAN REGIONAL MEDICAL CENTER, INC. Doing Business As: DUKE HEALTH LAKE NORMAN HOSPITAL Mooresville, NC Vitamin D 25 Hydroxy | $229.77 | $851.00 | $247.64–$293.60 | 73% | — source file |
| DUKE HEALTH LAKE NORMAN REGIONAL MEDICAL CENTER, INC. Doing Business As: DUKE HEALTH LAKE NORMAN HOSPITAL Mooresville, NC Vitamin D 25 Hydroxy | $229.77 | $851.00 | $247.64–$293.60 | 73% | — source file |
| MUSC Chester Regional Medical Center Chester, SC HB VITAMIN D25 HYDROXY | $279.50 | $559.00 | — | 50% | Apr 1, 2026 source file |
| MUSC Chester Regional Medical Center Chester, SC HB 25-HYDROXY D3 25HDN MA (SEND OUT) | $279.50 | $559.00 | — | 50% | Apr 1, 2026 source file |
| Gaston Memorial Hospital Mount Holly, NC CHG VITAMIN D 25 HYDROXY | $281.00 | $281.00 | — | — | May 11, 2026 source file |
| Gaston Memorial Hospital Gastonia, NC CHG VITAMIN D 25 HYDROXY | $281.00 | $281.00 | $222.55–$266.95 | — | May 11, 2026 source file |
| Gaston Memorial Hospital Belmont, NC CHG VITAMIN D 25 HYDROXY | $281.00 | $281.00 | $222.55–$266.95 | — | May 11, 2026 source file |
| Amisub of South Carolina, Inc. Rock Hill, SC VITAMIN D/25 HYDROXY/2 | $394.50 | $526.00 | $1.00–$526.00 | 25% | Sep 1, 2026 source file |
| Amisub of South Carolina, Inc. Fort Mill, SC VITAMIN D/25 HYDROXY/2 | $394.50 | $526.00 | $1.00–$526.00 | 25% | Apr 15, 2026 source file |
| Amisub of South Carolina, Inc. Rock Hill, SC VITAMIN D/25 HYDROXY | $417.00 | $556.00 | $1.00–$556.00 | 25% | Sep 1, 2026 source file |
| Amisub of South Carolina, Inc. Fort Mill, SC VITAMIN D/25 HYDROXY | $417.00 | $556.00 | $1.00–$556.00 | 25% | Apr 15, 2026 source file |
| MUSC Lancaster Medical Center Lancaster, SC HB 25-HYDROXY D3 25HDN MA (SEND OUT) | $1,014.00 | $2,028.00 | — | 50% | Apr 1, 2026 source file |
| MUSC Lancaster Medical Center Lancaster, SC HB VITAMIN D25 HYDROXY | $1,014.00 | $2,028.00 | — | 50% | Apr 1, 2026 source file |
Inpatient: lowest $153.75, median $224.53, highest $1,014.00 — a 6.6× difference within the same market.
As an outpatient, the same code is billed by 16 facilities here, median $224.53 — that is the price to compare if you are not being admitted.
Cash or insurance?
At 11 of 13 hospitals that publish insurer rates for this code here, the cash price is below the highest rate a health plan has negotiated, and at 1 it is below every plan's rate. If you have insurance but have not met your deductible, you usually pay your plan's negotiated rate, which falls somewhere in the "Insurers pay" column. Paying cash can cost less, but a cash payment usually does not count toward your deductible. Ask the hospital for both numbers before you book.
What it is
This blood test measures 25-hydroxy vitamin D, the form doctors use to judge whether the body has enough vitamin D. It is ordered to check for deficiency, for example with bone loss, certain digestive conditions or while taking supplements.
What the price covers
The price usually covers the vitamin D test only. The blood draw is often billed separately.