Vitamin D test cost in Denver, CO — inpatient
In Denver-Aurora-Centennial, CO, 10 hospitals list a cash price for vitamin D blood test, 25-hydroxy (the standard vitamin D level test) as an inpatient: from $31.66 to $1,093.47, with a median of $254.17. The lowest listed price is at Denver Health and Hospital Authority — $222.51 less than the median here. Across Colorado, the median is $54.82 at 17 hospitals.
Cash prices at 10 facilities across 6 cities for code 82306, as an inpatient (admitted to hospital) . Collected Sep 23, 2026; the hospital files themselves were last updated between Apr 1, 2026 and Jun 25, 2026.
Where each hospital's price falls
Each dot is one hospital's cash price on a scale from the lowest to the highest in Denver-Aurora-Centennial, CO.
Hospitals, cheapest first
| Hospital | Cash price | List price | Insurers pay | Off list | File date |
|---|---|---|---|---|---|
| Denver Health and Hospital Authority lowest Denver, CO · (303) 436-4927 HC VIT D3, 25-OH | $31.66 | $90.44 | $31.02 | 65% | Apr 30, 2026 source file |
| Childrens Hospital Colorado Aurora, CO · (720) 777-1234 HB=CSHB VITAMIN D (25 HYDROXY) | $37.05 | $57.00 | $8.55–$52.44 | 35% | Apr 17, 2026 source file |
| National Jewish Health Denver, CO · (303) 388-4461 HC VITAMIN D 25 HYDROXY | $41.34 | $59.05 | $5.16–$62.16 | 30% | — source file |
| Denver Health and Hospital Authority Denver, CO · (303) 436-4927 HC 25-HYDROXYVITAMIN D2 AND D3 BY TANDEM MASS SPECTROMETRY, SERUM ARUP 2002348 | $58.86 | $168.17 | $57.68 | 65% | Apr 30, 2026 source file |
| Denver Rehabilitation Hospital Denver, CO Vitamin d 25 hydroxy | $69.56 | $69.56 | — | — | Apr 1, 2026 source file |
| Reunion Rehabilitation Hospital Inverness Englewood, CO Vitamin d 25 hydroxy | $69.56 | $69.56 | — | — | Apr 1, 2026 source file |
| Childrens Hospital Colorado Aurora, CO · (720) 777-1234 HB=25-HYDROXYVITAMIN D2/D3 SERUM. | $83.20 | $128.00 | $19.20–$117.76 | 35% | Apr 17, 2026 source file |
| Childrens Hospital Colorado Aurora, CO · (720) 777-1234 HB=25-HYDROXYVITAMIN D2 AND D3 SERUM-ARUP | $85.15 | $131.00 | $19.65–$120.52 | 35% | Apr 17, 2026 source file |
| Childrens Hospital Colorado Aurora, CO · (720) 777-1234 HB=CALCIFEROL VITAMIN D | $141.70 | $218.00 | $32.70–$200.56 | 35% | Apr 17, 2026 source file |
| Childrens Hospital Colorado Aurora, CO · (720) 777-1234 HB=VITAMIN D 25OH | $158.60 | $244.00 | $36.60–$224.48 | 35% | Apr 17, 2026 source file |
| Childrens Hospital Colorado Aurora, CO · (720) 777-1234 HB=CSHB 25 HYDROXYVITAMIN D | $215.80 | $332.00 | $49.80–$305.44 | 35% | Apr 17, 2026 source file |
| AdventHealth Parker Parker, CO · (303) 269-4000 HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $438.77 | $438.77 | — | — | Apr 1, 2026 source file |
| AdventHealth Parker Parker, CO · (303) 269-4000 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY - ARUP | $438.77 | $438.77 | — | — | Apr 1, 2026 source file |
| AdventHealth Castle Rock Castle Rock, CO · (720) 455-5000 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $438.77 | $438.77 | — | — | Apr 1, 2026 source file |
| AdventHealth Castle Rock Castle Rock, CO · (720) 455-5000 HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $438.77 | $438.77 | — | — | Apr 1, 2026 source file |
| AdventHealth Castle Rock Castle Rock, CO · (720) 455-5000 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY - ARUP | $438.77 | $438.77 | — | — | Apr 1, 2026 source file |
| AdventHealth Porter Denver, CO · (303) 778-1955 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $438.77 | $438.77 | — | — | Apr 1, 2026 source file |
| AdventHealth Porter Denver, CO · (303) 778-1955 HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $438.77 | $438.77 | — | — | Apr 1, 2026 source file |
| AdventHealth Porter Denver, CO · (303) 778-1955 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY - ARUP | $438.77 | $438.77 | — | — | Apr 1, 2026 source file |
| AdventHealth Littleton Littleton, CO · (303) 730-8900 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $438.77 | $438.77 | — | — | Apr 1, 2026 source file |
| AdventHealth Littleton Littleton, CO · (303) 730-8900 HC VITAMIN D2 AND D3 25 HYDROXY LEVEL - ARUP | $438.77 | $438.77 | — | — | Apr 1, 2026 source file |
| AdventHealth Littleton Littleton, CO · (303) 730-8900 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY - ARUP | $438.77 | $438.77 | — | — | Apr 1, 2026 source file |
| AdventHealth Parker Parker, CO · (303) 269-4000 HC VITAMIN D 25-HYDROXY LEVEL DEFICIENCY | $438.77 | $438.77 | — | — | Apr 1, 2026 source file |
| Craig Hospital Englewood, CO HC VITAMIN D 25 HYDROXY | $1,093.47 | $2,870.00 | $1,486.66–$2,583.00 | 62% | Jun 25, 2026 source file |
Inpatient: lowest $31.66, median $254.17, highest $1,093.47 — a 34.5× difference within the same market.
As an outpatient, the same code is billed by 10 facilities here, median $254.17 — that is the price to compare if you are not being admitted.
Cash or insurance?
At 3 of 4 hospitals that publish insurer rates for this code here, the cash price is below the highest rate a health plan has negotiated, and at 1 it is below every plan's rate. If you have insurance but have not met your deductible, you usually pay your plan's negotiated rate, which falls somewhere in the "Insurers pay" column. Paying cash can cost less, but a cash payment usually does not count toward your deductible. Ask the hospital for both numbers before you book.
What it is
This blood test measures 25-hydroxy vitamin D, the form doctors use to judge whether the body has enough vitamin D. It is ordered to check for deficiency, for example with bone loss, certain digestive conditions or while taking supplements.
What the price covers
The price usually covers the vitamin D test only. The blood draw is often billed separately.